膀胱癌中MCM5和PCNA基因的表达及与临床病理因素的关系

Expressions of MCM5 and PCNA in the Bladder Tumor and Their Relationship with Clinical Pathological Parameters

目的:研究膀胱癌发生发展过程中微小染色体维持蛋白5(MCM5)和细胞增殖核抗原(PCNA)表达的意义及其相互关系。方法:采用免疫组织化学S-P法分别检测30例膀胱正常黏膜及膀胱癌标本中MCM5和PCNA的表达情况。结果:①MCM5和PCNA在膀胱由正常向癌转变的过程中,表达逐渐升高(P<0.01);②MCM5和PCNA呈正相关(相关系数rs=0.922,P=0.000)。结论:①MCM5和PCNA在人膀胱癌组织中蛋白的表达上调;②MCM5和PCNA在人膀胱癌组织中蛋白的表达与癌细胞分化程度和临床病理分期(UICC)密切相关;③MCM5和PCNA在膀胱癌变过程中能很好地反映细胞的增殖状况。

Objective：To study the significance of minichromosome maintenance 5（MCM5） and proliferating cell nuclear antigen（PCNA） protein expression in the bladder tumor and their relation.Methods：The MCM5 and PCNA protein of the bladder tumor were detected by immunohistochemical S-P.The relationships between MCM5 and PCNA expression and clinicopathological parameters were analyzed. Results：①The expressions of MCM5 and PCNA were gradually increased in the course of the bladder carcinomatous conversion（P〈0.01）;② Which were positively related to their （rs = 0. 922, P = 0. 000）. Conclusion： （1）Up - regulated expression of MCM5 and PCNA are obviously in human bladder tumor; （2）Expression level of MCM5 and PCNA protein in human bladder tumor tissue is correlated with differentiation degree of cancer cell and UICC; （3）MCM5 and PC- NA can well reflect cell proliferation in the course of the bladder carcinogenesis.