摘要
研究了以聚乙烯亚胺(PEI)为骨架复合高迁移率族蛋白B1(HMGB1)的复合型载体HMGB1/PEI的性能,以期提高非病毒基因载体的转染效率。透射电镜观察pDNA/HMGB1/PEI复合物粒子形态呈球形;动态光散射法测定粒径与表面电位,结果显示复合HMGB1后,复合物粒径降低,且随HMGB1加入量的增大表面电位有增大的趋势;凝胶电泳阻滞试验表明HMGB1可协助PEI与pDNA结合;MTT试验结果显示HMGB1/PEI复合载体的细胞毒性低于PEI;HMGB1/PEI复合载体的转染率较PEI的转染率增大2.9~4.0倍,且HMGB1可以弱化血清对转染的阻碍作用。所以HMGB1被证实能有效提高PEI的体外转染效率。
This study investigates the properties of a combined carrier, which is comprised of polyethylenimine (PEI) and a nuclear peptide, high mobility group box 1 (HMGB1), in order to obtain higher transfection efficiency of non-viral carriers. The pDNA/HMGB1/PEI combined polyplexes are spherical particles observed by transmission electron microscopy. Compared with using PEI alone, improved HMGB1 complexation is observed for smaller particle size of polyplexes and higher surface charge by dynamic light scattering. Gel electrophoresis retardation assays show that HMGB1 could help PEI to bind and condense pDNA. The results of cell viability by MTT assays suggest lower cytotoxicity for HMGB1/ PEI than PEI. The transfection efficiencies for pDNA/HMGB1/PEI combined polyplexes show more than 2. 9 -4. 0 times higher than for pDNA/PEI polyplexes, moreover, HMGB1 could weaken the inhibitory effects of serum on complexes. Thus, the combined carrier HMGB1/PEI which consists of the cationic polymer and the nuclear peptide is proved to enhance transfection efficiency of PEI in vitro.
出处
《中山大学学报(自然科学版)》
CAS
CSCD
北大核心
2009年第5期91-96,共6页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金资助项目(30600789)
广东省自然科学基金资助项目(5300666)