美洲大蠊提取物体外抑制牙龈卟啉单胞菌的生长

Extracts of Periplaneta americana inhibiting growth of Porphyromonas gingivalis in vitro

目的研究美洲大蠊提取物对体外培养的牙龈卟啉单胞菌的抗菌活性。方法以牙龈卟啉单胞菌ATCC33277为实验菌株,对倍稀释法测定美洲大蠊提取物的最低抑菌质量浓度(MIMC)和最低杀菌质量浓度(MBMC);在不同质量浓度的美洲大蠊提取物的牛脑心浸液培养液中培养牙龈卟啉单胞菌,分别于培养0、4、8、12、24和48h时测定细菌悬液的光密度值(D590)。结果美洲大蠊提取物对牙龈卟啉单胞菌的MIMC和MBMC分别为2.5g·L-1和5g·L-1,对牙龈卟啉单胞菌生长的抑制作用随其质量浓度的增加而增强。结论美洲大蠊提取物对体外培养的牙龈卟啉单胞菌的生长有抑制作用,其可能具有较强的防治牙周疾病的作用。

Objective To study the inhibitory effects of the extracts of Periplaneta americana on the growth of Porphyromonas gingivalis in vitro. Methods Porphyromonas gingivalis ATCC33277 was chosen as the experimental bacterium. The extracts of Periplaneta americana was double diluted to different concentrations by two-fold dilution, its effect on Porphyromonas gingivalis was measured by the minimal inhibitory mass concentration （MIMC）, the minimal bactericidal mass concentration （.MBMC） and D390 value of different culture medium at different times （0, 4, 8, 12, 24, 48 h）. Results The MIMC and MBMC of the extracts of Periplaneta americana was 2.5g·L^-1 and 5g·L^-1, respectively. The extracts of Periplaneta americana inhibitted multiplication of Porphyromonas gingivalis significantly and the effects became stronger with concentration increasing. Conclusion The antibacterial activity of the extracts of Periplaneta americana on Porphyromonas gingivalis was significant, indicating that the ex2 tracts of Periplaneta americana can be used as an agent for prevention of periodontal disease.