胃癌组织α-连接蛋白与幽门螺杆菌感染的相关性研究

The relationship of Helicobacter pylori infection and the expression of alpha-catenin in gastric cancer

目的探讨胃癌发生过程中α-连接蛋白(alpha-catenin,α-Cat)表达与幽门螺杆菌(helicobacter pylori,Hp)感染的关系,及其在胃癌发生发展中的可能机制。方法选择胃黏膜活检及手术切除的胃癌病理标本96例,癌旁组织96例,对照标本15例。应用免疫组化SP法检测各组组织中α-Cat的表达。用快速尿素酶试验或组织学Warthin-Starry嗜银染色法检测胃黏膜Hp感染情况。结果α-Cat在胃癌组、癌旁组织组、对照组的阳性表达率分别为34.4%、56.2%、100.0%,胃癌组显著低于癌旁组织组和对照组(P<0.05);96例胃癌组织中Hp阳性组α-Cat表达明显低于Hp阴性组(46.3%vs59.2%,P<0.05);α-Cat阳性率在高、中分化和低、未分化型胃癌组织中阳性率呈现递减趋势(69.1%vs10.7%,P<0.01),但α-Cat表达和淋巴结转移、癌肿浸润深度无密切相关。结论在胃黏膜癌变过程中,α-Cat的表达逐渐上调。胃癌组织中α-Cat的表达与Hp感染相关,提示Hp感染可通过对α-Cat表达的影响,在胃癌的发生发展过程中发挥一定作用。

Objective To investigate the expression and the clinical significance of Alpha-Catenin （α-Cat） in carcinogenesis of gastric mucosa and its relationship with Hehcobacter pylori （Hp） infection. Methods SP immunohistochemical technique was used to detect the expression of α-Cat in 96 cases of gastric carcinoma tissue and their adjacent tissues, and 15 control tissues. Rapid urease test and histological staining Warthin-Starry were used to determine the status of Hp infection. Results The positive rates of α-Cat expression in gastric cancer tissues,cancer-adjacent tissues and control tissues were 34. 4% ,56. 2% and 100% respectively （P 〈0. 05）. The expression of α-Cat in the Hp-positive cancer tissues was markedly lower than that in Hpnegative ones （36. 2% vs. 51.8% ,P 〈0. 05）. The positive rate of α-Cat in cancer tissue was positively paralleled with the increase of differentiation grade, but there was no significant difference between the positive rate of α-Cat and the depth of tumor invasion or lymph node metastasis （ P 〉 0. 05 ）. Conclusions The expression of α-Cat was gradually down-regulated during the course of gastric carcinogenesis. Hp infection is closely related to the expression of α-Cat in gastric cancer. Hp infection might be an initiating factor,and the activation of α-Cat might be one of the key events in the pathogenesis of gastric cancer. Hp infection and α-Cat expression play synergic roles in occurrence and development of gastric cancer.