多发性骨髓瘤患者p15、p16基因甲基化及其与预后的相关性

Relationship between methylation of p15/p16 genes and prognosis of multiple myeloma

目的探讨p15、p16基因的高甲基化与多发性骨髓瘤（MM）的发病和预后之问的关系。方法采用巢式甲基特异性PCR法（nMSP）检测47例MM患者p15、p16基因的甲基化状态，并对患者的临床资料及预后因素进行分析。结果47例MM患者p15、p16基因的甲基化比例分别为59．57％（28／47）、57．45％（27／47），两者同时存在甲基化者23例（48．94％）。Ⅱ、Ⅲ期MM患者p15、p16基因甲基化率明显高于Ⅰ期患者。结论p16、p15基因的高甲基化与MM的发病及预后相关。

Objective To illustrate the role of hypermethylation of p15 and p16 genes in the pathogenesis and prognosis of muhiple myeloma （MM）. Methods Forty-seven patients with muhiple myeloma were tested for methylation status of p15, p16 genes by nested-methylation specific PCR （n-MSP）, and the clinical data and prognostic factors of the 47 patients were analyzed. Results Twenty-eight （59.57 %） eases of methylation of p15 gene were observed, and twenty-seven （57.45 %） cases of methylation of p16 gene were observed, p15, p16 genes methylations were frequently detected in cases of stage Ⅱ and Ⅲ MM than stage Ⅰ MM. Conclusion There was a strong correlation between methylation of p15 and p16 gene, which might be related with the pathogenesis and prognosis of MM.