HIF-1α、PTEN在多发性骨髓瘤患者骨髓组织中的表达

Expression of HIF-1α and PTEN in human multiple myeloma bone marrow

目的研究多发性骨髓瘤（MM）骨髓组织中缺氧诱导因子1α（HIF—1α）和第10号染色体同源丢失性磷酸酶一张力蛋白基因（PTEN）的表达，探讨HIF-1α、PTEN在MM发病中的作用。方法应用SP免疫组织化学法检测28例初诊MM患者骨髓活检组织塑料包埋切片中HIF—1α和PTEN的蛋白表达。结果初诊MM患者骨髓组织中HIF-1α的阳性表达率为71．4％（20／28），与对照组的16．5％比较，差异有统计学意义（P〈0．01）；初诊MM患者骨髓组织中PTEN阳性表达率为42．9％（12／28），与对照组的100％比较，差异有统计学意义（P〈0．01）。初诊MM患者骨髓组织中HIF—1α与PTEN的表达呈中等强度负相关性（rn=-0．542，P〈0．05）。结论抑癌基因PTEN的缺失或表达下调及H1F-1α的过度表达可能在MM的发生、发展、浸润等生物学行为中起重要的作用，有可能作为临床监测MM进展的指标之一。

Objective To investigate HIF-loL and PTEN expression in multiple myeloma （MM）. Methods The expression of HIF-1α and PTEN was studied in 28 cases of MM with immunohistochemistry. Results Over-expression of HIF-1α was frequently （20 of 28 cases, 71.4 %） detected in MM bone marrow. Expression of HIF-1α between MM and control group bone marrow has significant differenees（P 〈0.01）. The positive expression rate of PTEN in MM bone marrow was 42.9 %（12 of 28 cases）. Expression of PTEN between MM and control group bone marrow has significant differences （P 〈0.01）. There was negative correlation between the expression of HIF-1α protein and the PTEN protein （P 〈0.05, rn=-0.542）. Conclusion The absence or low expression of PTEN and the increased levels of HIF-1α may play a critical role in the formation, development and invasion of MM. HIF-1α and PTEN may be used to estimate the progress of MM.