海湾扇贝外套膜创伤修复过程的组织学和组织化学观察

Histology and Histochemistry in Mantle Wound Repairing of Bay Scallop

Ｍａｌｏｒｙ＇ｓ三色染色显示海湾扇贝外套膜创伤的组织学修复过程。外套切割３小时、６小时，伤口处涌出血淋巴细胞，有非颗粒变形细胞与颗粒变形细胞两种构成。切割２４小时伤口内出现大量的成纤维细胞；到切割后８天，伤愈组织内出现少量成肌细胞；１６天，愈合物内肌纤维增多，并可见到血窦形成；切割３２天，肌纤维含量丰富，但这些肌纤维并未形成明显的肌肉束。组织化学观察表明，高碘酸—Ｓｃｈｉｆ反应发现血淋巴细胞质内糖元含量高。由成纤维细胞向成肌细胞过渡期糖元稍有增加。而经茚三酮—Ｓｃｈｉｆ反应显示伤愈过程中各种细胞蛋白质含量稳定。通过ｆｕｌｇｅｎ－ｓｃｈｉｆ反应与花青染色比较显示伤愈组织内成纤维细胞ＲＮＡ合成活跃。

Mallory's trichrome and histochemical stain methods were carried out in mantle wound sections of bay scallop.Within 6 hours after incision,Large clumps of lymphocytes appeared along the wound surface.By 24 hours incision,collagen-like fibers had been formed among the wounded muscle and the fibroblast cells occured in the reparative tissue.Myoblast cells appeared by 8 days after wounding,its number rose after 16 and 32-day incision. In addition,There is glycoen in the lymphocytes.which increased slightly from fibroblast to myoblast cells.There is no protein variation in reqenerating process.Moreover,RNA synthesis raised in the fibroblast cells.