抗、感小麦赤霉病品种RFLP的初步分析

RFLP Analysis of Wheat Varieties Resistant or Susceptible to Scab

为选择遗传多样性大的小麦抗、感赤霉病亲本构建作图群体，对４个感病品种（“Ａｌｏｎｄｒａ”、“中国春”、“宁麦３号”、“扬麦３号”）和４个抗病品种（“望水白”、“苏麦３号”、“８９４０３７”、“８９５００４”）进行了ＲＦＬＰ分析。用３０个探针进行检测，其中８个在抗／感组合间侦察到多态性。以望水白／Ａｌｏｎｄｒａ、苏麦３号／Ａｌｏｎｄｒａ、８９４０３７／Ａｌｏｎ－ｄｒａ这３个组合的ＲＦＬＰ频率最高，适合构建分离群体进行抗病基因的定位。现已构建了这３个组合的重组自交系群体，达Ｆ５代，对其多态性检测正在进行中。已检测的９８个探针，在上述３个组合中的多态性频率分别达到３０．６％、２８．６％和２４．５％。还发现探针ＫＳＵＦ１１与ＥｃｏＲＩ、ＨｉｎｄⅢ和ＢａｍＨＩ的酶切片段的杂交图谱上，在抗病品种望水白、苏麦３号及抗病突变体８９４０３７、８９５００４中各有１条谱带；在感病品种中则无此带，推测可能与抗赤霉病有一定的相关性。

In order to select one most divergent pair of parents for a mapping population,RFLP was used to detect polymorphisms among eight wheat varieties,including four accessions(“Alondra”,“Chinese Spring”,“Ningmai 3”,“Yangmai 3”)susceptible and four accessions(“Wangshuibai”,“Sumai 3”,“894037”,“895004”)resistant to scab.The results showed that the combinations of Wangshuibai/Alondra,Sumai 3/Alondra,894037/Alondra had the highest polymorphisms and were suitable for constructing a mapping population.Now the recombinant inbred line(RIL)populations of these three crosses have advanced to F 5 generations with a total of 98 RFLP probes tested,the frequencies of the polymorphism were 30 6%, 28 6%,24 5% in above three mentioned combinations,respectively.When probe KSUF11 was hybridized to the genomic DNA digested with EcoRI,HindⅢ,BamHI,one specific band was observed in all four resistant varieties,but absent in susceptible cultivars,indicating the probe may be related to scab resistance.Two somaclonal variants were also identified with RFLP in this experiment.