摘要
以京欣一号、查理斯顿、巨人、井神小西瓜、黑崩筋５个品种苗龄６ｄ的子叶为外植体，建立了西瓜品种无性繁殖系和高效的培养体系，在ＭＳ＋ＢＡ５ｍｇ／Ｌ＋ＩＡＡ０．５ｍｇ／Ｌ培养基上，芽诱导率为４６．６％～９４％，转接到附加ＫＴ０．２ｍｇ／Ｌ的ＭＳ培养基上进行再生芽的伸长培养后，在ＭＳ＋ＮＡＡ０．５ｍｇ／Ｌ的根诱导培养基上的诱导率达９６．２％。
An effective system for vegetative propagation and culture has been successfully set up by using cotyledons from five watermelon(Citrullus vulgaris) cultivars.High frequency shoot regeration(46 6—94%) was obtained from 6 day old cotyledons of cultivars cultured on medium containing MS+BA5mg/L+IAA0 5 mg/L.Buds elongated on MS medium containing KT 0 2mg/L.The MS medium with 0 1mg/L NAA was used for rooting and the rate of root induction was also above 80%.
出处
《华北农学报》
CSCD
北大核心
1998年第3期112-115,共4页
Acta Agriculturae Boreali-Sinica
关键词
西瓜
子叶
组织培养
植株再生
Watermelon
Cotyledon
Tissue culture
Plant regeneration
