加拿大金露梅离体培养与快繁体系的建立

Culture in Vitro and Rapid Propagation System with Potentilla fruiticosa 'Gold Drop'

以加拿大金露梅嫩芽、叶片为外植体进行试验,通过观察确定嫩芽为初代培养的外植体。运用L9(34)正交试验筛选出最适合的腋芽初代培养、芽苗继代增殖及组培苗生根的最佳培养基,从而为金雨点建立了一套"腋芽诱导—继代增殖—生根培养"的快速繁殖体系。结果表明,诱导腋芽的分化培养基为MS+6-BA 2 mg.L-1+NAA 0.1 mg.L-1,其中MS、6-BA和NAA均为诱导的主要因子,影响顺序为MS>6-BA>NAA,诱导率达96.33%;继代增殖培养中激素6-BA是影响加拿大金露梅芽增殖生长的主要激素,差异极显著(p=0.000 1),并且以浓度为2.0 mg.L-1的增殖效果最好,确定最佳培养基为MS+6-BA 2.0 mg.L-1+NAA 0.6 mg.L-1+KT 0.2mg.L-1,半月增殖倍数达6.12;对生根培养3种激素(KT、NAA、IBA)进行方差分析,KT和NAA的影响均达到了显著水平(p=0.000 1;0.001 0),是影响生根的主导因子,而IBA的p值为0.424 5,因此可以忽略其影响,在诱导时确定用NAA和KT两种激素。之后对生根数量、生根率进行多重比较表明最终确定生根培养基为MS+NAA0.1 mg.L-1+KT 1.0 mg.L-1,生根数为8.63条,生根率为95.33%。

The buds of Potentilla fruiticosa ＇Gold Drop＇ was chosen the adapted primarily culture explants by experiment. The best medium was screened for axillary budprimarily culture, bud seedling subculture multiplication and tissue culture shoot rooting by L9 （3^4） orthogonal experiment. The rapid propagation system of P.fruiticosa ＇Gold Drop＇, axillary bud induction-subculture multiplication-rooting culture, was formed. The result showed The differentiation medium of axillary bud induction was MS ＋ 6-BA 2 mg·L^- 1 ＋ NAA 0.1 mg·L^- 1. MS, 6-BA and NAA was the main factors and MS 〉6-BA 〉 NAA. The induction rate was reached to 96.33%. 6-BA was the principal hormone that affects the proliferation of P.fruiticosa buds which showed a significant dis- crepancy（p =0. 000 1 ）, and the reproduction effect was the best when the concentration was 2.0 mg·L^-1. MS ＋ 6-BA 2.0 mg·L^-1＋ NAA 0.6 mg·L^-1 ＋ KT 0.2mg·L^-1 were defined as the optimum mediums with the multiples of proliferation in 15 days reaching 6.12. KT and NAA were the main factors on affecting rooting by variance analysis and IBA was no influencing. So KT and NAA were chosen for inducing hormone. The optimal rooting medium was MS ＋ NAA 0.1 mg·L^-1＋ KT 1.0 mg·L^-1 by multiple comparison. The rooting number was 8.63 and rooting rate reached to 95.33%.