放射线对口腔鳞状细胞癌细胞中核因子-κB p65表达的影响

Effected of radioactive ray on the expression of NF-κB p65 in human oral squamous cell carcinoma

目的:探讨不同剂量放射线对口腔鳞状细胞癌(OSCC)细胞中核因子-κB(nuclear factor-κB,NF-κB)p65信号转导蛋白表达的影响。方法:采用人舌鳞状细胞癌Tca8113细胞,分为6个剂量组,分别为0y、2、4、6、8、10Gy,在照射后的不同时间点(1,3,6,10,24,48h)应用免疫细胞化学检测NF-κB p65的表达情况,应用Leica Qwin Plus图像分析系统对图像进行分析,测其灰度值,并应用SPSS10.0软件对数据进行X2检验。结果:免疫细胞化学方法显示不同剂量组的平均灰度值与0Gy组相比均为P<0.05,差异均具有统计学意义,而不同时间点组的平均灰度值相互比较,3h组的平均灰度值与其他时间点组相比均为P<0.05,差异有显著性。结论:放射线可以激活NF-κB p65信号转导通路,此信号转导通路在肿瘤的放射治疗中可能具有重要意义。

Objective：To investigate the effected of radioactive ray on the expression of NF-κB p65 in human oral squamous cell carcinoma. Method：To adopt the squamous cell carcinoma of tongue Tca8113 cell,the experiment was divided into six groups,0、2、4、6、8 and 10 Gy group. To use immunocyteochemistry to detect the NF-κB p65 protein after irradiation in various times（1、3、6、10、24、48 h） .To apply the Leica Qwin Plus image analytical system to analyzed the gray scale. Result：To compare the brtween various dose group and 0 Gy group ,the P 〈0.05,the difference was statistical significance.The difference of average gray scale between 3h group and other different time group was significance （P 〈0.05）. Conclusion： Radioactive ray can activitate the NF-κB p65 of oral squmaous cell carcinoma cell.NF-κB p65 maybe an important activity in the tumor radiotherapy.