维生素E对醛固酮诱导的大鼠心肌重构及氧化应激的干预作用

Vitamin E reduces the oxidative stress and myocardial remodeling induced by aldosterone

目的:观察外源性醛固酮(aldosteronre,Ald)灌注对大鼠心肌重构和氧化应激水平的影响,以及抗氧化剂维生素E(VitE)的干预作用。方法:大鼠随机分为:①对照组,②Ald组,③Ald+VitE组和④Ald+螺内酯(spironolactone,Spi)组。用黄嘌呤氧化法测定超氧化物歧化酶的活性,用巴比妥酸比色法测定丙二醛的含量,Masson三色法检测心肌胶原,细胞凋亡原位检测法检测心肌细胞凋亡。结果:与Ald组比较,Ald+VitE组心肌胶原、凋亡指数、丙二醛明显减少,超氧化物歧化酶明显增加(P<0.05)。Ald+VitE组与Ald+Spi组上述指标差异无统计学意义。结论:VitE能够降低Ald诱导的大鼠心肌氧化应激水平,显著改善心肌纤维化和心肌细胞凋亡。

Objective：To investigate the oxidative stress and myocardial remodeling induced by aldosterone （Ald） in rats and the intervention of Vitamin E （Vit E）. Method：Rats were divided into 4 groups randomly： ①Control group,②Ald group,③Ald＋VitE group,④Ald＋Spi group. The SOD and MDA were detected by zanthine hydrocarbonylation and malonylurea colourinetry,respectively.Cardiac collagen and apoptosis were detected by Masson trichromatism and TUNEL methods,respectively. Result：Compared with Ald group,SOD increased,MDA,cardiac collagen and AI decreased significantly in Ald＋VitE group. There was no significant difference between Ald＋VitE and Ald ＋ Spi group. Conclusion：Vitamin E can reduce oxidative stress,myocardial fibrosis and apoptosis induced by aldosterone and improve myocardial remodeling in rats.