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转基因动物在microRNA研究中的应用 被引量：6

The Application of Transgenic Animals in MicroRNA Research
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摘要 MicroRNA是一类在转录后水平上调节基因表达的非编码小分子RNA,在生物体生理、病理等过程中发挥重要作用.MicroRNA功能的研究将是未来人们关注的焦点.通过转基因技术建立的多种动物模型在整体水平揭示了基因的功能.近年,以microRNA为研究对象的转基因动物模型数量不断增加,构建策略不断丰富.通过miRNA过表达、敲除及敲减等手段已揭示了miRNA在肿瘤、心血管系统疾病等多方面的作用.转基因动物正成为microRNA研究中不可或缺的工具. MicroRNAs （miRNAs） are small non-coding RNAs which are essential for posttranscriptional gene regulation and have important roles in physiology and pathology. The researches on function of miRNA will be a focus in the future. Several animal models have been built by transgenic technology, making important contributions to our understanding of gene function at the whole scale. Recently, the number of transgenic animal models for microRNAs and construction strategies have been increasing and diversifying. Some roles ofmiRNA in tumor and cardiovascular disease have been revealed by transgenic animals. Transgenic animals are becoming a kind of powerful tool in microRNA researches.
作者 马宁 高旭
机构地区 哈尔滨医科大学基础医学院
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2009年第9期1095-1100,共6页 Progress In Biochemistry and Biophysics
关键词 MICRORNA 转基因动物 基因打靶 转基因 microRNA, transgenic animal, gene targeting, transgene
分类号 Q52 [生物学—生物化学]
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参考文献48

  • 1Scholten J, Hartmann K, Gerbaulet A, et al. Mast cell-specific Cre/loxP-mediated recombination in vivo. Transgenic Res, 2008, 17 (2): 307-315.
  • 2Dann C T. New technology for an old favorite: lentiviral transgenesis and RNAi in rats. Transgenic Res, 2007, 16 (5): 571 -580.
  • 3Burket C T, Montgomery J E, Thummel R, et al. Generation and characterization of transgenie zebrafish lines using different ubiquitous promoters. Transgenic Res, 2008, 17(2): 265 -279.
  • 4Sokol N S, Xu P, Jan Y N, et al. Drosophila let-7 microRNA is required for remodeling of the neuromusculature during metamorphosis. Genes Dev, 2008, 22(12): 1591 - 1596.
  • 5Palmiter R D, Brinster R L, Hammer R E, et al. Dramatic growth of mice that develop from eggs microinjected with metallothioneingrowth hormone fusion genes. Nature, 1982, 222(615): 611 -615.
  • 6Vervoort V S, Lu M, Valencia F, et al. A novel Flk1-TVA transgenic mouse model for gene delivery to angiogenic vasculature. Transgenic Res, 2008, 17(3): 403-415.
  • 7Christen U, von Herrath M G. Transgenic animal models for type 1 diabetes: linking a tetracycline-inducible promoter with a virus-inducible mouse model. Transgenic Res, 2002, 11 (6): 587 595.
  • 8Riazi A M, Van Arsdell G, Buchwald M. Transgenic expression of CECR1 adenosine deaminase in mice results in abnormal development of heart and kidney. Transgenic Res, 2005, 14 (3): 333-336.
  • 9Guillemot F, Cerutti I, Auffray C, et al. A transgenic mouse model engineered to investigate human brain-derived neurotrophic factor in vivo. Transgenic Res, 2007, 16(2): 223-237.
  • 10Lee R C, Feinbaum R L, Ambros V. The C. elegans hereochronic gene lin-4 encodes small RNAs with antisence complementarity to lin-14. Cell, 1993, 75(5): 843-854.

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生物化学与生物物理进展
2009年 第9期

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