Netherton综合征及红斑型天疱疮表皮中激肽释放酶相关肽酶和丝氨酸蛋白酶抑制物的检测

Detection of KLKs and Serpins in the Epidermis of Netherton Syndrome and Pemphigus Erythematosus

目的探讨激肽释放酶相关肽酶家族(KLKs)和丝氨酸/半胱氨酸蛋白酶抑制物(Serpins)在Netherton综合征(NS)及红斑型天疱疮(PE)患者表皮中的表达。方法选用LEKTI,SLPI,KLK5,KLK7的羊抗人多克隆抗体,以免疫组织化学结合图像分析软件进行半定量分析的方法研究NS,PE患者表皮中KLK5,KLK7,LEKTI,SLPI等的表达情况。结果NS组表皮中KLK5,KLK7,SLPI平均光密度分别高于对照组141.49%,523.88%,134.20%。PE组的KLK5,KLK7,SLPI平均光密度分别高于对照组90.73%,456.42%,77.11%。结论NS和PE表皮中KLK5,KLK7升高,而SLPI则代偿性上调。

Objective To investigate the characteristics of KLKs and Serpins in the epidermis of Netherton syndrome （NS） and pemphigus erythematosus （PE） patients. Methods The expression of LEKTI, SLPI, KLKS, KLK7 were studied in the epidermis of NS and PE with immnnohistochemistry and semi-quantitative analysis with image-pro plus 6.0. Results the mean density of KLKS, KLK7, SLPI in NS group was higher than that in control group by 141.49%, 523.88%, 134.20% respectively. In PE group, the mean density of KLKS, KLK7, SLPI was 90.73%, 456.42%, 77.11% higher than that in control group. Conclu- sion Expression level of KLK5 and KLK7 increased in NS and PE. and SLPI increased as well.