氟伐他汀对系统性硬皮病成纤维细胞增殖及胶原分泌的影响

Effect of fluvastatin on the proliferation of fibroblasts and collagen secretion in systemic sclerosis

目的:确定氟伐他汀对系统性硬皮病(systemic scleroderma,SS)皮肤成纤维细胞(fibroblasts,Fb)增殖及胶原分泌的影响。方法:原代培养SS患者皮肤Fb 72 h,四甲基偶氮唑盐(MTT)比色法检测不同浓度的氟伐他汀对细胞的存活力,并绘制不同浓度氟伐他汀作用下Fb的生长曲线;观察不同浓度的氟伐他汀对Fb胶原分泌的影响。结果:与空白对照组相比,各浓度的氟伐他汀均明显抑制患者皮肤Fb的增殖,使细胞的生长速度减慢,显著减少Fb培养液中可溶性胶原的含量,差异有显著性(P<0.05),且随着药物浓度的增高而增强,具有明显的浓度依赖性。结论:氟伐他汀对SS皮肤Fb的增殖及胶原的分泌均有显著抑制作用,为其治疗硬皮病提供了理论基础。

Objective： To determine the effect of fluvastatin on the proliferation of human skin fibroblasts and collagen secretion in systemic scleroderma （SS）. Methods： Human skin fibroblasts were cuhm-ed. The potential of cell proliferation at different concentrations of fluvastatin was detected by the tetrazohum dye colorimetric test （ MTT Test）. The growth curve of fibroblasts was drawn with MTT method. The collagen was detected by bio - chemical method. Results： Fluvastatin inhibited the proliferation of the cultured dermal fibroblasts of SS and reduced the syn- thesis of collagen in a dose - dependent manner. The difference was significant compared with controls （ P 〈 0.05）. Conclusion： Fluvastatin can both inhibit the proliferation of skin fibroblasts and reduce the collagen secretion of dermal fibroblasts, which forms the base in the treatment of systemic scleroderma.