上皮生长因子受体-信号转导与转录激活因子3信号转导通路与喉乳头状瘤的相关性研究

Study on the correlation between EGFR-STAT3 signal pathway and laryngeal papilloma

目的:探讨喉乳头状瘤中上皮生长因子受体(EGFR)和信号转导与转录激活因子3(STAT3)的表达与其生物学行为的关系。方法:采用RT-PCR、免疫组织化学方法和Westernblot,检测42例喉乳头状瘤和15例正常喉黏膜组织中EGFR和STAT3、磷酸化-STAT3(p-STAT3)的mRNA和蛋白质表达情况,同时将蛋白质表达水平与喉乳头状瘤的临床病理参数进行相关性分析。结果:EGFR与STAT3的mRNA在喉乳头状瘤组织表达水平显著高于正常喉组织(P<0.05,P<0.01)。免疫组织化学及Westernblot方法也证实喉乳头状瘤组织中EGFR与STAT3(p-STAT3)的蛋白质表达水平显著高于正常喉组织(P<0.05)。EGFR与STAT3(p-STAT3)蛋白质在喉乳头状瘤组织的表达显著关联(P<0.05),STAT3蛋白与喉乳头状瘤的复发和恶变有明显相关性(P<0.05)。结论:EGFR-STAT3信号转导通路参与喉乳头状瘤的发生,STAT3蛋白的持续活化可能促进喉乳头状瘤的复发与恶变。

Objective：To explore the relationship between the expression of EGFR and STAT3 in human laryngeal papilloma and its biological behavior. Method：Reverse transcription polymerasechain reaction（RT-PCR）, immunohistochemical staining and Western blot were used to evaluate the mRNA and protein expression of EGFR and STAT3（p-STAT3） in 42 laryngeal papilloma tissues and 15 samples of normal laryngeal tissue, and the relationship between the protein expression of them and clinicpathological parameters was also analysized. Result： The mRNA expression levels of EGFR and STAT3 in laryngeal papilloma tissue were significantly higher than that in normal laryngeal tissue（P〈0.05 ,P〈0.01）. Protein positive expression of EGFR and p-STAT3 were also detected in a significantly greater proportion of laryngeal papilloma than normal laryngeal tissue by immunohistochemistry and western blot（P〈（0.01 ,P〈0. 05）. There was relationship between EGFR and p-STAT3 overexpression in laryngeal papilloma（P〈0.05）. The expression p-STAT3 was correlated with the recurrence and canceration of laryngeal papilloma（P〈0.05）. Conclusion： The EGFR-STAT3 signal transduction pathway may be involved in the pathogenesis of laryngeal papilloma,, and the persistent activation of STAT3 gene plays an important role in the recurrence and canceration of laryngeal papilloma.