雷公藤甲素对ERα蛋白介导的人乳腺癌MCF-7细胞增殖抑制的影响

Proliferation inhibition effects of triptolide on estrogen receptor （ER）α protein-mediated MCF-7 human breast cancer cells

目的：研究雷公藤甲素对激素依赖性人乳腺癌细胞MCF-7增殖的抑制作用及可能的机制。方法：MTT法检测雷公藤甲素对MCF-7细胞增殖的抑制作用，Hoechst染色法检测其对细胞凋亡的影响，Western blotting法观察雷公藤甲素对雌激素受体（ER）α蛋白表达的影响。结果：雷公藤甲素对MCF-7细胞增殖有显著的抑制作用，并且呈现良好的时效和量效关系，雷公藤甲素能有效诱导MCF-7细胞凋亡，并且对ERα具有明显的下调作用。结论：雷公藤甲素能抑制人乳腺癌细胞MCF-7增殖并且诱导其凋亡，其诱导MCF-7细胞凋亡的作用机制可能与其下调ERα的表达有关。

AIM： To research the proliferation inhibition effect and possible mechanism of triptolide on estrogen-sensitive MCF-7 human breast cancer cells. METHODS： The inhibition effect of triptolide on MCF-7 was examined using MTT assay, the apoptotic effect induced by triptolide was investigated by Hoechst technique, and the expression of estrogen receptor （ER）α was also observed with Western blotting. RESUITS： The inhibition effect induced by triptolide on MCF-7 cell was obvious and in a concentration and time dependent manner. Triptolide could efficaciously induce the cell apoptosis and down-regulate the expression of ERα in MCF-7 cells. CONCLUSION： Triptolide could inhibit human breast cancer cell MCF-7 proliferation and induce its apoptosis, the mechanism of apoptosis may be associated with the down-regulation of the ERa expression.