拓扑异构酶Ⅰ在人病理性瘢痕组织中的表达分析

Expression of topoisomerase Ⅰ in human pathological scar tissues

背景：有研究表明，DNA拓扑异构酶Ⅰ（TopoⅠ）在多种肿瘤细胞内的含量明显高于正常组织。目的：通过免疫组织化学染色方法比较病理性瘢痕与正常皮肤组织中DNA拓扑异构酶Ⅰ差异。设计、时间及地点：对比观察，于2005-01/2007-12在南昌大学第一附属医院烧伤科完成。材料：人病理性瘢痕组织标本来同期南昌大学第一附属医院手术切取的人病理性瘢痕组织21例，男15例，女6例；同一患者手术多余的正常皮肤组织标本21例作为对照。方法：对人病理性瘢痕和正常皮肤进行苏木精-伊红染色，鉴定是否为病理性瘢痕和正常皮肤；以免疫组织化学染色检测DNA拓扑异构酶Ⅰ在角质形成细胞和成纤维细胞中的表达。按拓扑异构酶Ⅰ染色阳性细胞占全部细胞的百分比分4个等级：≤5%为阴性（－）；6%~24%为弱阳性（＋）；25%~49%为阳性（＋＋）；≥50%为强阳性（＋＋＋）。主要观察指标：人病理性瘢痕和正常皮肤标本拓扑异构酶Ⅰ在角质形成细胞和成纤维细胞中的表达。结果：拓扑异构酶Ⅰ在人病理性瘢痕和正常皮肤组织的角质形成细胞，成纤维细胞中均有表达。正常皮肤中，角质形成细胞及成纤维细胞各有3例免疫组织化学阳性；病理性瘢痕组织中，角质形成细胞及成纤维细胞各有7例和12例呈阳性。正常皮肤角质形成细胞拓扑异构酶Ⅰ阳性表达率为14.3%，病理性瘢痕组织表达率为33.3%，差异无明显不同（P=0.147 3）；正常皮肤成纤维细胞拓扑异构酶Ⅰ阳性表达率为14.3%，病理性瘢痕阳性总表达率为57.1%，存在明显差异（P=0.003 8）。结论：在病理性瘢痕组织成纤维细胞中，拓扑异构酶Ⅰ的表达明显高于正常皮肤成纤维细胞。

BACKGROUND： Study shows that many kinds of tumour cells show much more DNA topoismerase （TopoⅠ） than normal tissues. OBJECTIVE： To compare the difference in DNA TopoⅠexpression between pathological scar and normal skin tissues through immunohistochemical staining. DESIGN, TIME AND SETTING： A contrast observation was completed in the Burn Center, the First Affiliated Hospital of Nanchang University from January 2005 to December 2007. MATERIALS： Human pathological scar samples were surgically taken from 21 patients （15 male, 6 female） of the First Affiliated Hospital of Nan-Chang University. The extra normal tissues samples cut off from the 21 patients were taken as the controls. METHODS： Haematoxylin-eosin staining was performed to the human pathological scars and the normal skins to verify their authenticity; Immunohistochemical staining was used to determine the expression of DNA TopoⅠin keratinocytes and collagenoblasts. Four levels were classified according to the percentage of positive TopoⅠin all cells： negative（-） when ≤ 5%, weakly positive （＋） when 6%-24%, positive （＋＋） when 25%-49%, and strongly positive （＋＋＋） when 50%. MAIN OUTCOME MEASURES： DNA TopoⅠexpression in keratinocytes and collagenoblasts of human pathological scar and normal skin samples. RESULTS： TopoⅠwere expressed in keratinocytes and collagenoblasts of both human pathological scar and normal skin. In normal skin, 3 cases showed positive immunohistochemical staining results in both keratinocytes and fibroblasts; In pathological scar tissues, 7 positive were found in karytinocytes and 12 positive were found in fibroblasts. The positive expression percentage of TopoⅠin keratinocytes was 14.3% in normal skin and 33.3% in pathological scar, which showed there was no significant difference between the two （P =0.147 3）. The positive expression percentage of TopoⅠin fibroblasts was 14.3% in normal skin and 57.1% in pathological scar, which showed there was significant difference between the two （P =0.003 8）. CONCLUSION： The expression of TopoⅠin fibroblasts of pathological scar is significantly higher than that of normal skin.