gr/gr-DAZ1/DAZ2基因缺失与男性不育

Deletion of gr/gr-DAZ1/DAZ2 gene and male infertility

目的研究中国人群中gr/gr-DAZ1/DAZ2缺失与男性不育的关系。方法应用PCR和RFLP分析的方法,在364例男性不育患者和287个正常男性中,对gr/gr-DAZ1/DAZ2基因缺失进行调查。结果在男性不育患者和正常男性中均发现gr/gr-DAZ1/DAZ2基因缺失,但男性不育患者中的缺失频率显著高于正常男性(9.3%vs2.8%,P<0.001)。结论gr/gr-DAZ1/DAZ2基因缺失不是中国人群男性不育的直接致病遗传因素,而是一个发病的风险因子,尚不足以作为男性不育基因诊断的指标。

Objective To study the relationship between the gr/gr-DAZ1/DAZ2 gene deletion and male infertility in Chinese population. Methods Distribution of gr/gr-DAZ1/DAZ2 gene deletion was investigated in 364 infertile men and 287 fertile controls using polymerase chain reaction（PCR） and fragment length polymorphism analysis （RFLP）. Results gr/gr-DAZ1/DAZ2 gene deletion was detected in both infertile men and controls, but the incidence of gr/ gr-DAZ1/DAZ2 gene deletion in infertile men was significantly higher than that in controls （9. 3% vs 2. 8%, P 〈 0. 001 ）. Conclusion Deletion of gr/gr-DAZ1/DAZ2 gene is not a direct mechanism but a risk factor of male infertility, suggesting that it is not necessary to detect this deletion in gene diagnosis for male infertility among Chinese.