摘要
目的探讨C-JUN激活区结合蛋白-1(Jab1)与P27kip1在卵巢癌细胞株HO-8910中的表达变化及相互关系。方法用血清饥饿及饥饿后释放处理HO-8910细胞,分别用Western blot及核浆分离技术检测Jab1、P27kip1表达及亚细胞定位;用免疫沉淀方法检测Jab1与P27kip1的结合情况;脂质体介导法将Jab1基因转染HO-8910细胞,Westernblot及核浆分离检测P27kip1表达。结果血清饥饿导致HO-8910细胞生长周期停滞,P27kip1蛋白总量增加,且主要集中分布于胞核;Jab1蛋白总量减少,主要表达于核质;血清释放后两者呈现相反的表达变化,但是P27kip1主要集中分布在胞质;P27kip1与Jab1在HO-8910细胞中存在相互结合的情况。脂质体转染Jab1后,P27kip1表达下降且定位有明显的胞质改变。结论Jab1可能通过与P27kip1结合来介导P27kip1出核并影响其表达,从而参与调控HO-8910细胞的生长。
Objective To investigate the expression and relationship of Jab1 and P27klp1 in ovarian cancer cell HO- 8910. Methods Cells were treated with serum starvation and release. The expression and distribution of Jab1 and P27^kip1 were detected by western blot and subcellular fractionation. HO-8910 cells were transfected in vitro with pcDNA3.1-MYC-Jab1. Western blot as well as subcellular fractionation was used to detect the expression and localization of P27^kip1. Results The growth of HO-8910 cells was blocked by serum starvation. P27^kip1 increased while Jab1 decreased. The reverse changes were found after serum release. P27^kip1 and Jab1 could form compound in HO- 8910 cells detected by immunoprecipitation. 48h after transfected by pcDNA3.1-MYC-Jab1, the expression of P27^kip1 decreased and the distribution of P27^kip1 translocated from nucleus into cytoplasma in HO-8910. Conclusion Jab1 may control the location and expression of P27^kip1 through integrating with P27^kip1 , and participate in regulating the growth of ovarian cancer cells through interfering with the function of P27^kip1.
出处
《基础医学与临床》
CSCD
北大核心
2009年第9期955-960,共6页
Basic and Clinical Medicine
