期刊文献+

基因工程技术在生物制药领域的应用和发展 被引量:5

Application and development of genetic engineering technology in biopharmaceutics
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摘要 本文简述了近年来基因工程在生物制药技术的发展和应用。其中主要从基因操作中大分子的分离、PCR技术、基因芯片、外源基因的表达这4个方面叙述基因工程相关技术的应用和发展,以及基因工程药物的产业化现状与发展趋势。 This review focus on the perspective of genetic engineering technology in biopharmaceutical field. Four general aspects of genetic engineering are further discussed in this review as following: the separation of maeromolecules, PCR, gene chip, and the expression of foreign genes. The large-scale or industrialization of genetic engineering technology in biopharmaceutics is also reviewed.
出处 《基础医学与临床》 CSCD 北大核心 2009年第9期996-998,共3页 Basic and Clinical Medicine
基金 江苏省高技术研究计划医药资助(BG20070605)
关键词 PCR技术 基因芯片 外源基因的表达 产业化 PCR gene chip expression of recombinant genes industrialization
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参考文献10

  • 1宋现让,柳永蕾,刘贤锡,魏玲,王兴武.质粒载体系统介导的人肿瘤细胞RNA干扰作用[J].基础医学与临床,2005,25(9):807-812. 被引量:7
  • 2Tougan T, Okuzaki D, Nojima H. Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification [ J ]. Nucleic Acids Research, Online, 2008, 36: e92.
  • 3Haddad F, Qin AX, Giger JM, et al. Potential pitfalls in the accuracy of analysis of natural sense-antisense RNA pairs by reverse transcription-PCR [J]. BMC Biotechnology, 2007, 7:21 -35.
  • 4邓雪柯,殷建华,曹毅.3种5’RACE技术的比较与优化[J].成都医学院学报,2007,2(1):20-25. 被引量:12
  • 5江禾,王友群.基因芯片技术与药物的研究和开发[J].世界临床药物,2008,29(2):104-107. 被引量:3
  • 6Ho Y, Lo HR, Lee TC, et al. Enhancement of correct protein folding in vivo by a non-lytic baeulovirus [ J ]. Biochem, 2004, 382: 695- 702.
  • 7Calleja V, Alcor D, Laguerre M, et al. Intramolecular and Intermolecular Interactions of Protein Kinase B Define Its Activation in vivo [J]. PLoS Biol, 2007, 5:780-791.
  • 8Xu XH, Meier-Schellersheim M, Yah JS, et al. Locally controlled inhibitory mechanisms are involved in eukaryotic GPCR-mediated chemosensing [ J ]. J Cell Biol, 2007, 178 : 141 - 153.
  • 9丁锡申.重组蛋白类药物[J].中国生物工程杂志,2005,25(4):78-78. 被引量:1
  • 10袁志刚,张进平,王缨,储以微,徐薇,熊思东.真核化的原核表达系统增强HBV preS2/S基因免疫的效果[J].中国免疫学杂志,2007,23(1):6-10. 被引量:1

二级参考文献32

  • 1王广良,陈成彬,高建明,倪虹,王同顺,陈力.细胞周期相关基因微阵列在中药抑制肝癌细胞增殖作用机理研究中的应用[J].中国中药杂志,2005,30(1):50-54. 被引量:13
  • 2Scherr M, Morgan MA, Eder M. Gene silencing mediated by small interfering RNAs in mammalian cells[J]. Curr Med Chem, 2003,10(3): 245-256.
  • 3Kim VN. RNA interference in functional genomics and medicine[J]. J Korean Med Sci, 2003, 18(3): 309-318.
  • 4Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells[J]. Nature, 2001, 411(6836): 494-498.
  • 5Caplen NJ, Parrish S, Imani F, et al. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems[J]. PNAS, 2001, 98(17):9742-9747.
  • 6Milhavet O, Gary DS, Mattson MP. RNA interference in biology and medicine[J]. Pharmacol Rev, 2003, 55(4): 629-648.
  • 7Sui G, Soohoo C, Affar el B, et al. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells[J]. PNAS, 2002, 99(8): 5515-5520.
  • 8Yu JY, DeRuiter SL, Turner DL. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells[J]. PNAS, 2002, 99(9): 6047-6052.
  • 9Paddison PJ, Caudy AA, Bernstein E, et al. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells[J].Genes Dev, 2002, 16(8): 948-958.
  • 10Tran N, Cairns MJ, Dawes IW, Arndt GM. Expressing functional siRNAs in mammalian cells using convergent transcription[J]. BMC Biotechnol, 2003, 3(1): 21-29.

共引文献19

同被引文献38

  • 1黄雅琼,石德顺.基因工程药物的研究热点及发展方向[J].玉林师范学院学报,2009,30(5):67-69. 被引量:3
  • 2林丽彬,林建银.内皮抑素及其抗血管生成作用[J].福建医科大学学报,2007,41(2):189-190. 被引量:5
  • 3Robert M, Sjin TT, Satchi-Faninaro R, et al. A 27-aminoacid synthetic peptide corresponding to the NH2-terminal zinc-binding domain of endostatin is responsible for its antitumor activity [ J ]. Cancer Res, 2005, 65 (9) : 3656 - 3663.
  • 4Hurrell J. Monoclonal hybridoma antibodies: techniques and applications [ M ]. Elorid : CRC Press, 1982:49 - 51.
  • 5Jiang YP, Pannell R, Liu JN, et al. Evidence for a novel binding protein to urokinase-type plasminogen activator in platelet membranes [ J ]. Blood, 1996, 87 ( 7 ) : 2775 - 2781.
  • 6Chiou GCY. Ophthalmic toxicology (target organ toxicology series) [ M ]. 2nd ed, Philadephia : Taylor & Francis, 1999 : 189 - 191.
  • 7O' Reilly MS, Boehm T, Shing Y, et al. Endostatin : An endogenous inhibitor of angiogenesis and tumor growth [ J ]. Cell, 1997, 88(2): 277-285.
  • 8Lee TY, Robert M, Sjin TT,et al. Linking antibody Fc domain to endostatin significantly improves endostatin half-life and efficacy[ J]. Clin Cancer Res, 2008, 14(5) : 1487 - 1493.
  • 9Boehem T, Folkman J, Browder T, et al. Antiangiogentic therapy of experimental cancer does not induce acquired drug resistance[ J]. Nature, 1997, 390:404 -407.
  • 10Chura-Chambi RM, Genora LA, Affonso R, et al. Refolding of endostatin from inclusion bodies using high hydrostatic pressure [ J ]. Anal Biochem, 2008, 379 ( 1 ) : 32 - 39.

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