大口黑鲈内含子1上SNP G208A的CRS-PCR检测方法

Establishment of CRS-PCR Method of Genotyping SNP G208A in the Intron 1 of the Largemouth bass(Micropterus salmoides)

创造限制酶切位点PCR法是应用引物错配技术结合单核苷酸多态性(SNP)而配合成一个酶切位点,使SNP可用于PCR-RFLP分析的一种方法。应用CRS-PCR技术检测了大口黑鲈(Micropterus salmoides)IGF-I基因内含子1上SNPG 208A的多态性,并详述了CRS-PCR错配引物的设计方法,CRS-PCR引物设计和应用的注意事项,以促进CRS-PCR单核苷酸多态检测方法在水产动物研究领域的应用。

Created restriction site PCR （CRS-PCR） uses mismatches in one of the two PCR primers flanking the single nucleotide polymorphism to create or remove a restriction endonuclease recognition site in order to identify SNP genotypes. In this study, the genotype of SNP G208A in the intron 1 of the largemouth bass was identified with method of CRS-PCR. In addition, it was described a web-based program that facilitates the design of mismatched PCR primers to create or remove a restriction endonuclease recognition site and considerations for CRS-PCR primer design. The web-based program advanced the application of CRS-PCR in aquatic molecular breeding.