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斑马鱼crip2基因的克隆、亚细胞定位及在胚胎发育早期的表达分析

The cloning, subcellular localization and expression of zebrafish crip2 in early embryo
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摘要 为揭示包含具有LIM结构域的crip基因的功能,以斑马鱼为实验对象,克隆了斑马鱼的crip2基因,并用全胚胎原位杂交法检测crip2在斑马鱼胚胎中的表达,用亚细胞定位法检测crip2基因在细胞中的定位.研究发现crip2基因在斑马鱼胚胎早期没有表达,五体节开始在脊索特异表达;后期,主要局限于在血管、心脏和体节间肌肉,表明crip2的表达并不局限于内皮系统.在细胞内,crip2基因在细胞核和胞浆中均有表达. The spatial and temporal expression pattern of crip2 in zebrafish was investigated. The full length cDNA of zebrafish crip2 was cloned. By subcellular localization,the crip2 protein was located in both the nucleus and endochylema. By whole-mount in situ hybrization, the expression of crip2 was not detected until 5-somite stage, when it started to express specificly in the notochord. Then its expression mainly limited to the blood vessel, the heart and the inter-somite muscle, indicating that the distribution of crip2 transcript was not limited to the endothelial system.
作者 解丽芳 刘昌永 姚少华 杨寒朔
机构地区 四川大学生命科学学院四川大学华西医院生物治疗国家重点实验室
出处 《四川大学学报（自然科学版）》 CAS CSCD 北大核心 2009年第5期1515-1519,共5页 Journal of Sichuan University(Natural Science Edition)
基金 国家重点基础研究发展规划(2004CB518800)
关键词 crip2基因 斑马鱼 全胚胎原位杂交 亚细胞定位 crip2, zebrafish, whole-mount in situ hybridization, subcellular localization
分类号 Q344 [生物学—遗传学]
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参考文献17

  • 1Baltz R, Evrard J, Domon C, et al. A LIM motif is present in a pollen-specific protein[J]. Plant Cell, 1992,4:1465.
  • 2Muller L,Xu G,Wells R,et al. LRG1 is expressed during sporulation in Saccharomyces cerevisiae and contains motifs similar to LIM and rho/racGAP domains [J]. Acids Res, 1994,22:3151.
  • 3Wu R Y,Gill G N. LIM domain recognition of a tyrosine-containing tight turn[J]. Biol Chem, 1994,269: 25085.
  • 4Birkenmeier E H, Gordon J I. Developmental regulation of a gene that encodes a cysteine-rich intestinal protein fingers are at identical positions in both the human and maps near the murine immunoglobulin heavy chain locus, and rat proteins and, while the S3N1 zinc finger differs [J]. Proc Natl Acad Sei, 1986,83:2516.
  • 5Hallquist N A, Khoo C, Cousins R J. Lipopolysaeh eharide regulates cysteine-rieh intestinal protein, a zinc-finger protein,in immune cells and plasma [J]. Leukocyte Biol, 1996, 59 : 172.
  • 6Miyasaka K Y,Kida Y S, Sato T,et al. Csrpl regulates dynamic cell movements of the mesendoderm and cardiac mesoderm through interactions with Dishevelled and Diversin [J]. Proc Natl Acad Sci USA,2007,104(27) :11274.
  • 7Joyce J A, Laakkonen P, Bernasconi M, et al. Stagespecific vascular markers revealed by phage display in a mouse model of pancreatic islet tumorigenesis[J]. Cancer Cell, 2003,4 : 393.
  • 8Levenson C W, Shay N F, Lee-Ambrose L M, et al. Regulation of cysteine-rich intestinal protein by dexamethasone in the neonatal rat [J]. Proc Natl Acad Sci,1993,90:712.
  • 9Hallquist N A,Khoo C,Cousins R J. Lipopolysac charide regulates eysteine-rich intestinal protein,a zinc-- finger protein,in immune cells and plasma [J]. Leukocyte Biol, 1996,59 : 172.
  • 10Cousins R J, Lanningham-Foster L. Regulation of cysteine-rich intestinal protein, a zinc finger protein,by mediators of the immune response[J]. J Infect Dis, 2000,182 (suppl 1), 82.

二级参考文献12

  • 1Hardiman G. Introduction to proteomics:tools for the new biology. Expert Rev Proteomics,2004;1(1) :9-10.
  • 2Tay TL, Lin Q, Seow TK, et al. Proteomic analysis of protein profiles during early development of the zebrafish, Danio rerio. Proteomies ,2006 ;6(10) :3176-3188.
  • 3Denslow ND, Chow MC, Kroll KJ, et al. Vitellogenin as a biomarker of exposure for estrogen or estrogen mimics. Ecotoxicology,1999;8(5):385-398.
  • 4Verleyen P, Baggerman G, Wiehart U, et al. Expression of a novel neuropeptide, NVGTLARDFQLPIPNamide, in the larval and adult brain of Drosophila melanogaster. J Neurochem,2004 ;88(2) :311-319.
  • 5Schulz-Knappe P, Zucht HD, Heine G, et al. Peptidomics:the comprehensive analysis of peptides in complex biological mixtures. Comb Chem High Throughput Screen, 2001;4 (2): 207-217.
  • 6Svensson M, Skold K, Svenningsson P, et al. Peptidomicsbased discovery of novel neuropeptides. J Proteome Res,2003; 2(2) :213-219.
  • 7Schulte I, Tammen H, Selle H, et al. Peptides in body fluids and tissues as markers of disease. Expert Rev Mol Diagn, 2005 ; 5 (2) : 145-157.
  • 8Hummon AB, Amare A, Sweedler JV. Discovering new invertebrate neuropeptides using mass spectrometry. Mass Speetrom Rev,2006;25(1) :77-98.
  • 9Adermann K, John H, Standker L, et al. Exploiting natural peptide diversity: novel research tools and drug leads. Curr Opin Biotechnol, 2004 ; 15 (6) : 599-606.
  • 10Schoofs L, Baggerman G. Peptidomics in Drosophila melanogaster. Brief Funct Genomic Proteomic, 2003; 2 (2): 114-120.

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四川大学学报（自然科学版）
2009年 第5期

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