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异形胞发育的蓝细菌Dna A蛋白的表达研究

The study of expression of Dna A in heterocyst developmental cyanobacterium
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摘要 构建了Anabaena PCC7120的复制起始蛋白Dna A的重组表达质粒,在Escherichiacoli中诱导其超量表达,纯化后免疫家兔获得抗体,采用western blotting检测Dna A在营养细胞和异形胞中的表达情况.结果显示,两种不同类型的细胞中都能够检测到Dna A的表达,而且在不同缺氮诱导时间,异形胞中Dna A的表达存在差异.说明DNA复制起始与异形胞的发育可能存在着一定的关系. The corresponding antiserum against Dna A proteins of Anabaena PCC7120 were collected by injecting rabbits with purified proteins after over-expression from recombinant expressing plasmid in Escherichia coli. Then the antiserum was used to detect the expression of Dna A in vegetative cell and heterocyst by Western blotting. The result showed that Dna A could be detected in both type cells, and moreover, the expression of Dna A is different in different period heterocysts induced with nitrogen stepdown. It indicated certain relations exist between DNA replication and heterocyst differentiation.
作者 魏新元 丑敏霞 陈文莉
机构地区 西北农林科技大学食品科学与工程学院 西北农林科技大学生命科学学院 华中农业大学生命科学与技术学院
出处 《四川大学学报（自然科学版）》 CAS CSCD 北大核心 2009年第5期1520-1524,共5页 Journal of Sichuan University(Natural Science Edition)
基金 陕西省自然科学基金(SJ08B12) 国家973计划子课题(2001CB1089)
关键词 蓝细菌 异形胞 DNA复制 DNA A 分化 cyanobacterium, heterocyst, DNA Replication, Dna A, differentiation
分类号 Q93 [生物学—微生物学] Q78 [生物学—分子生物学]
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参考文献22

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四川大学学报（自然科学版）
2009年 第5期

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