决明方对肥胖大鼠脂肪肝及其SREBP-1c表达的影响

Effect of Jueming prescription on the fatty liver and its SREBP-1c expression in the obese rats

目的:观察决明方对肥胖大鼠非酒精性脂肪肝的干预作用及对肝脏固醇调节元件结合蛋白-1c(SREBP-1c)表达的影响。方法:雄性SD大鼠随即分为对照组普通饲料喂养,模型组和决明方组高脂饲料喂养制备肥胖并发脂肪肝模型。8周后决明方组用决明方口服液治疗,模型组和对照组给予等量注射用水,持续4周。检测血脂、肝组织三酰甘油(TG)和肝功能,观察肝组织病理学改变,RT-PCR测定肝SREBP-1c及其靶基因脂肪酸合成酶(FAS)mRNA表达,Western Blot测定肝SREBP-1蛋白表达。结果:与模型组比较,决明方组肝TG和血清TG明显降低(P<0.01,P<0.05);血HDL-C明显升高(P<0.01);肝脂肪变性明显减轻;肝SREBP-1c及FAS的mRNA表达均明显下降(P<0.01);SREBP-1蛋白表达明显减少(P<0.01)。结论:决明方下调SREBP-1c及其靶基因的表达,对非酒精性脂肪肝有一定的治疗作用。

OBJECTIVE To study the therapeutic effect of Jueming prescription（JMP） on nonalcoholic fatty liver disease in obese rat model, and investigate its influence on the expression of sterol regulatory element binding-protein 1c（SREBP-1c） in the liver. METHODS Male SD rats were randomly divided into： normal control group fed with standard diet; model group and JMP group fed with high-fat diet. After 8 weeks, rats in JMP group were treated with JMP for 4 weeks. TG in the liver, the indexes of serum lipid and liver function were measured. The pathological changes of liver were observed. The mRNA expressing of SREBP-1c and its target gene fatty acid synthetase（FAS） in the liver were detected by RT-PCR. And the protein expression of SREBP-1 was detected with Western Blot. RESULTS Compared with model group, TG in the liver and serum were significantly decreased（P〈0. 01, P〈0. 05 respectively）, and serum HDL-C increased in JMP group. Meanwhile, the liver steatosis was ameliorated in JMP group. The mRNA expression of SREBP-1c and FAS in JMP group were markedly lowered than model group（P〈0. 01）. And protein expression of SREBP-1 was also decreased in JMP group（P〈0. 01）. CONCLUSION JMP can alleviate liver steatosis, which may be associated with down-regulation of the expression of SREBP-1c and its target gene in the fatty liver.