欧亚旋覆花总黄酮提取物对大鼠局灶性脑缺血再灌注损伤的保护作用研究

Protection by Total Flavonoids from Inula britannica against Focal Cerebral Ischemia-Reperfusion Injury in Rats

目的:研究欧亚旋覆花总黄酮提取物(TFIB)对大鼠局灶性脑缺血再灌注损伤的保护作用。方法:实验分为6组,即假手术、模型、金纳多及TFIB高、中、低剂量组。对各组大鼠进行神经功能评分,采用TTC染色法测定脑梗死体积、HE染色法观察脑组织形态学变化、黄嘌呤氧化酶法测定血清中超氧化物歧化酶(SOD)活性、硫代巴比妥法测定丙二醛(MDA)含量、双波长荧光分光光度法检测大鼠大脑皮层和纹状体神经细胞胞浆Ca2+浓度([Ca2+]i)的变化。结果:与模型组比较,TFIB高、中剂量组神经功能评分显著降低(P<0.01);TFIB高、中剂量组缺血再灌注损伤后脑梗死体积显著减小(P<0.01);TFIB高、中剂量组神经细胞变性坏死的病理变化显著改善;TFIB高、中剂量组大鼠血清SOD活力显著增强,MDA含量显著降低(P<0.01);TFIB高、中剂量组胞浆Ca2+浓度显著降低(P<0.01)。结论:TFIB对脑缺血再灌注损伤有显著的保护作用,其作用机制与抑制并减少体内脂质过氧化物的产生、增强机体抗氧化活性有关。

OBJECTIVE： To study the protection by the total flavonoids from Inula britannica（TFIB） against focal cerebral ischemia-reperfusion injury. METHODS： The experimental rats were divided into 6 groups： sham operation group, model group, Ginaton group, TFIB groups （high, medium and low dosage） .The neurological score of each group was evaluated, and the infarction size was measured by TTC staining; the histological changes of brain tissue were observed by HE staining, serum activity of superoxide dismutase （SOD） was detected by xanthinoxidase method; serum content of malondi- aldehyde （MDA） was determined by thio-barbitone method; the intracellular calcium concentration（[Ca^2＋]i） in cerebral cortex and striatum was determined by two wave - length fluorospectrophotometry. RESULTS： As compared with model group, the rats treated by TFIB （either high or medium dosage） had significantly decreased neurological scores（P 〈0.01）, significant improvement in pathological change of degeneration and necrosis of neurocytes and significant increase in SOD activity but significantly decrease in MDA content （P〈0.01）; TFIB groups （high, medium） showed significantly decreased cerebral infarction size after ischemical- reperfusion injury（P〈0.01） and significant decrease of the intracellular calcium concentration（[Ca^2＋]i） （P 〈 0.01） . CONCLUSION ： The remarkable protection by TFIB against focal cerebral ischemia - reperfusion injury might be attributed to the inhibition and reduction of the production of lipid peroxidative product in body and the enhancement of the antioxydant activity.