月见草油微球的体外释放度研究

In Vitro Release of Evening Primrose Oil Microspheres

目的:研究月见草油微球的体外释放特征。方法:采用紫外分光光度法测定微球在不同介质中的累积释放度,以零级、一级和Higuchi方程对释药行为进行拟合。结果:微球在人工胃肠液中连续溶出6h的累积释放度可达(92.84±0.35)%;以人工胃液为介质,药物释放符合Higuchi方程(Q=5.941t1/2—11.083,r=0.9917);以人工肠液为介质,药物释放符合一级速率方程(ln(100—Q)=—0.4874t+4.588,r=0.9964)。结论:该方法简单、准确、重现性好,可用于月见草油微球的体外释放度测定。

OBJECTIVE： To study the release characteristics of Evening primrose oil microspheres in vitro. METHODS： UV speetrophotometry was adopted to determine the accumulated release of microspheres under different media, and the drug release behaviors in vitro were fitted using zero-order, first-order and Higuchi equations. RESULTS： The accumulated release of microspheres in the gastrointestinal liquid （within 6 h） was up to（92.84±0.35） % ;under the media of artificial gastric juice, the release of drug conformed to Higuchi equation： Q = 5.941t^1/2-11.083（r = 0.9917）; under the media of artificial intestinal juice, the release of drug was in line with first- rate equation： In（100-Q） =-0.4874t ＋ 4.588（ r = 0.9964） . CONCLUSION： This method is simple, accurate and reproducible, and it can be used for the determination of the release rate of the Evening primrose oil microspheres.