RP-HPLC法同时测定欧亚旋覆花中4种黄酮的含量

Simultaneous Determination of 4 Flavonoids in Inula britannica by RP-HPLC

目的:建立以反相高效液相色谱法同时测定欧亚旋覆花中4种黄酮(槲皮素、木犀草素、菠叶素、6-甲氧基-木犀草素)含量的方法。方法:色谱柱为Inertsil ODS-3(250mm×4.6mm,5μm),柱温为40℃,流动相为甲醇-乙腈-0.49%枸橼酸(梯度洗脱),流速为1.0mL.min-1,检测波长为360nm。结果:4种黄酮化合物的进样量分别在0.17～6.8μg(r=0.9999)、0.088～3.52μg(r=0.9999)、0.0202～0.808μg(r=0.9999)、0.045～1.8μg(r=0.9996)范围内与各自峰面积积分值呈良好的线性关系;平均回收率在96.03%～99.78%之间,RSD在0.73%～1.36%之间(n均=6)。结论:本方法简单、灵敏、准确、重现性好,可用于欧亚旋覆花药材及其制剂的质量控制。

OBJECTIVE： To establish the RP - HPLC method for simultaneous determination of flavonoids（quercetin, cyanidenon, spinacetin, and 6 - methoxyl cyanidenon） in Inula britannica. METHODS： The samples were separated on chromatographic column Inertsil ODS- 3（250mm×4.6mm,5μm） at a column temperature of 40℃.The mobile phase consisted of methanol- acetonitrile- 0.49% citric acid（gradient elution） at a flow rate of 1.0 min·mL^-1. The detection wave- length was set at 360 nm. RESULTS： The linear ranges of quercetin, cyanidenon, spinaeetin, and 6-methoxyl- cyanidenon were 0.17-6.8μg（r = 0.9999）, 0.088-3.52μg（r = 0.9999）, 0.0202-0.808μg（r = 0.9999） and 0.045-1.8μg（r = 0.9996）, respectively, and the average recoveries of all stood at 96.03%- 99.78% and RSD at 0.73%-1.36% （n = 6 each）. CONCLUSION： The method is simple, sensitive, accurate and reproducible, and it is applicable for the quality control of the raw medicinal substance and preparation of I.britannica .