摘要
目的:建立以反相高效液相色谱法测定艾迪注射液中人参皂苷Rg1含量的方法。方法:色谱柱为汉邦Lichrospher C18(250mm×4.6mm,5μm),流动相为水-乙腈(梯度洗脱),流速为1.0mL.min-1,检测波长为203nm,柱温为35℃。结果:人参皂苷Rg1的检测浓度在5.0～250.0μg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9995);平均回收率为97.43%,RSD=1.81%(n=6)。结论:本方法简便、快速、准确,可用于艾迪注射液的质量控制。
OBJECTIVE： To establish the RP- HPLC method for determination of ginsenoside Rg1 in Aidi injection. METHODS： The determination was performed on Lichrospher C18（250mm×4.6mm,5μm） with column temperature kept at 35℃. The mobile phase consisted of water - aeetonitrile （gradient elution） at a flow rate of 1.0 mL·min^-1.The detection wavelength was set at 203 nm. RESULTS： The linear range of ginsenoside Rgt was 5.0-250.0μg·mL^-1（ r = 0.9995）, and the average recovery was 97.43% （RSD = 1.81%, n = 6）. CONCLUSION： The method is simple, rapid and accurate, and it is suitable for the quality control of Aidi injection.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第27期2131-2132,共2页
China Pharmacy
