期刊文献+

一种从血浆中分离纯化脂蛋白(a)的方法 被引量:1

A METHOD FOR SEPARATION AND PURIFICATION OF LIPOPROTEIN(A) FROM PLASMA
下载PDF
导出
摘要 高纯度的Lp(a)对于Lp(a)测定的标准化及生物学研究都是迫切需要的。本研究以连续的亲和层析、凝胶过滤层析、离子交换层析取代超速离心,建立了1种新的分离纯化Lp(a)的方法。结果表明:用该方法可以批量纯化Lp(a)。 Highly purified Lp (a) is required for standardization of measurement of Lp(a) and for biological research. In this study, a novel method,instead of ultracentrifugation, was established to separate and purify Lp(a) by sequential uses of affinity, size -exclusion and ion - exchange chromatography. The results indicated that Lp(a) was produced in large - scale using this method.
出处 《内蒙古农业大学学报(自然科学版)》 CAS 北大核心 2009年第2期1-4,共4页 Journal of Inner Mongolia Agricultural University(Natural Science Edition)
关键词 脂蛋白(a) 纯化 柱层析 Lipoprotein(a) purification column chromatography
  • 相关文献

参考文献8

  • 1Berg K. A new serum type system in man: the Lp system [J]. Acta Pathol Microbiol Scand, 1963, 59:362 -382.
  • 2Berglund L, Ramakrishnan R. Lipoprotein (a): an elusive cardiovascular risk factor [ J ]. Arterioscler Thromb Vasc Biol. 2004, 24(12) :2219 -2226.
  • 3Koschinsky ML, Marcovina SM. Lipoprotein (a) : structural implications for pathophysiology [ J ]. Int J Clin Lab Res. 1997,27 ( 1 ) : 14 - 23.
  • 4Sangrar W, Bajzar L, Nesheim ME, et al. Antifibrinlytic effect of recombinant apolipoprotein(a) in Vitro is primarily due to attenuation of tPA - mediated Glu - plasminogen activation [ J ]. Biochemistry, 1995,34 ( 15 ) : 5151 - 5157.
  • 5Xia J, May LF, Koschinsky ML. Characterization of the basis of lipoprotein [ a ] lysine - binding heterogeneity [J]. J Lipid Res. 2000, 41(10) :1578 -1584.
  • 6Fless GM, ZummaUen ME, Scanu AM. Physicochemical Properties of Apolipoprotein (a) and Lipoprotein ( a - ) Derived from the Dissociation of Human Plasma Lipoprotein (a) [J]. J Biol Chem, 1986, 261 (19) : 8712 - 8718.
  • 7Guevara J, Suurlino J, Jan AY, et al. Proposed mechanisms for binding of apo(a) kingle type 9 to apoB - 100 in human lipoprotein(a) [J]. Biophys J, 1993, 64(3) : 686 - 700.
  • 8Lippi G, Lo Caseio C, Ruzzenente O, et al. Simple and rapid procedure for the purification of lipoprotein (a) [J]. J Chromatogr B, 1996, 682(2) :225 -231.

同被引文献12

引证文献1

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部