H9N2亚型禽流感病毒A/Chicken/Guangdong/21/01株反向基因操作系统的建立

ESTABLISHMENT OF REVERSE GENETICS SYSTEM OF H9N2 AVIAN LNFLUENZA VIRUS A/CHICKEN/GUANGDONG/21/01

H9N2亚型禽流感病毒自1994年在中国大陆首次分离以来,不仅在家禽中广泛流行,而且也可以感染哺乳动物和人。由于H9N2亚型禽流感病毒是低致病性的,更增加了其感染人并在人群中存在下去的可能性,因此研究其感染哺乳动物的分子机制具有重要的意义。本研究建立了A/Chicken/Guangdong/21/01(简称:CK/GD/21)病毒的8质粒反向基因操作系统,并通过转染293T细胞成功拯救了该病毒R-GD/21。R-GD/21在对SPF鸡和BALB/c小鼠的致病性方面保持了与亲本野毒一致的生物学特性。CK/GD/21反向基因操作系统的成功建立,为今后通过基因替换、定点突变技术确定H9N2亚型禽流感病毒感染哺乳动物的分子机制奠定了基础。

H9N2 avian influenza viruses （AIV） have widely circulated in domestic poultry, even isolated from pigs and human since the first H9N2 AIV was isolated from chicken in China's Mainland. H9N2 AIVs are low pathogenicity influenza viruses, and enormously possible to caused disease and potential circulation in human. Thus it is very essential for us to study the mechanism of crossing the species barrier and infect mammalian. In the present study, we established an eight - plasmid reverse genetics system and rescued GD/ 21/01 （ R - GD/21/01 ） through 293T cell transfection. Animal studies showed that the rescued recombinant virus maintained the biological properties of the wild type GD/21/01 virus （ W - GD/21/01 ）. The successfully establishment of the reverse genetics system of GD/21/01 will enable us to conduct studies of the molecular basis of replication H9N2 avian inflection viruses in mammalian mouse model.