碳纳米管对大鼠呼吸系统的氧化损伤

Oxidative Damage of Respiratory System in Rats Exposed to Carbon Nanotubes

目的探讨碳纳米管(CNTs)对大鼠呼吸系统的氧化损伤。方法将12只Wistar大鼠随机分成染毒组和对照组,采用非暴露式气管滴注染毒方式,染毒剂量为22.5mg/kg,1次/d,连续15d。眼眶取血后处死大鼠,测定大鼠肺泡灌洗液(BLAF)和血清的氧化损伤指标。结果与对照组相比,染毒后大鼠体重有逐渐降低趋势,并且染毒停止3d后,体重下降的趋势仍在继续。染毒后大鼠BLAF及血清中SOD的活力均出现降低,但不明显(P>0.05);MDA含量则显著增高,差异有统计学意义(P<0.01);T-AOC的活力在染毒后大鼠血清及BLAF中均出现降低,差异有统计学意义(P<0.01);而AKP的活力变化不明显(P>0.05)。结论CNTs可引起大鼠呼吸系统的氧化损伤,脂质过氧化产物增多对机体产生毒性作用,机体总抗氧化能力有所下降。

Objection To explore the oxidative damage of respiratory system in rats exposed to carbon nanotubes （CNTs）. Methods Twelve Wistar rats were randomly divided into two groups （the treated group and the control group） and exposed to CNTs through intratraeheal instillation at the dose of 22.5 mg/kg （calculated with CNTs） once a day for 15 days, then the rats were sacrificed, the biochemicaly indexes in serum and broncboalveolar lavage fluid （BLAF） were measured. Results The results showed that CNTs could cause decrease of body weight gain, even after three days of stoping exposure to CNTs. Compared with the control group, the activity of superoxide dismutase （SOD） and total antioxidant capacity （T-AOC） in serum and BLAF of exposed rats decreased, the level of malondialdehyde （MDA） in serum and BLAF increased significantly. Conclusion CNTs exposure can induce the decrease of the antioxidant capacity and oxidative damage of respiratory system in rats.