脾虚大鼠中枢单胺类神经递质含量及四君子汤干预的研究

The research of spleen deficiency rats＇ monoamine neurotransmitter and the treatment mechanism of Si- junzi Decoction

目的研究脾虚大鼠中枢单胺类神经递质含量的变化和四君子汤干预的途径。方法将60只大鼠分为空白对照组、脾虚组、四君子汤组，每组各20只，使用高效液相检测大鼠纹状体中5-HT、DA、5-HIAA、DOPAC和HVA的含量。结果与对照组比较，脾虚组大鼠纹状体内5-HT（2．25±0．78）ng／mg水平显著升高，DA（26．06±9．01）ng／mg水平显著下降（P〈0．01）；脾虚大鼠使用四君子汤干预后，纹状体内5-HT（1．86±0．53）ng／mg和DA（38．95±10．86）ng／mg水平基本恢复正常，与对照组差异无显著性，而DA水平与脾虚组大鼠差异有显著性（P〈0．05）。结论脾虚证大鼠纹状体中的单胺类神经递质的含量异常，脾虚证存在有中枢机制，健脾名方四君子汤可以调节脾虚大鼠中枢单胺类神经递质含量。

Objective To analyze the change of spleen deficiency rats＇ monoamine neurotransmitter and the treatment mechanism of Sijunzi decoction. Methods 60 rats were divided into control group, spleen deficiency group and Sijunzi Decoction group. And every group had 20 rats. Then high performance liquid chromatograph was used to analyze rats＇ 5-HT, DA,5-HIAA, DOPAC and HVA,in their corpus striatum. Results Compared with control group,the concentration of 5-HT[ （2.25 ± 0.78 ） ng/mg ] in spleen deficiency rats significantly ascended, and DA [ （ 26.06 ± 9.01 ） ng/mg] significantly descended （P 〈 0.01 ）. After the treatment of Sijuuzi decoction, the level of 5-HT [ （ 1.86 ± 0.53 ） ng/mg ] and DA [ （ 38.95 ± 10.86 ） ng/mg ] came back to normal. And compared with spleen deficiency rats,treatment rats＇ DA significantly ascended （P 〈 0.05）. Conclusion There is a con- sanguineous relationship between spleen deficiency syndrome and rats＇ monoamine neurotransmitter, and spleen deficiency syndrome has a central mechanism. And Sijunzi decoction could be used to treat with spleen deficiency syndrome by regulating monoamine neurotransmitter.