水产品中三聚氰胺含量的快速检测方法研究

Research on the Rapid Detection Methods of Melamine Content in Fishery Products

[目的]参考国家标准建立水产品中三聚氰胺含量的检测方法。[方法]分别用高效液相色谱法和液相色谱/质谱联用法检测水产品样品液中的三聚氰胺含量。[结果]高效液相色谱法和液相色谱/质谱联用法都能较好地检测三聚氰胺含量,但其检测限不同。利用高效液相色谱法检测三聚氰胺含量的检测限为2.0 mg/kg,出峰时间为7.25 min。利用液相色谱/质谱联用法检测三聚氰胺含量的检测限为0.01mg/kg。高效液相色谱法检测三聚氰胺含量的浓度-峰面积曲线为:y=22.351 3+46.380 7x,R=0.998 4。液相色谱/质谱联用法检测三聚氰胺含量的浓度-峰面积曲线为:y=39.702 5+16.264 6x,R=0.995 7。[结论]液相色谱/质谱联用法的检测时间更短,灵敏度更高,检测效果更好,但检测成本较高。高效液相色谱法检测效果略差,但更经济实用。

The research aimed to establish the detection method of melamine content in fishery products with reference to national standard. [ Method] The melamine contents in the sample solutions of fishery products were detected by HPLC and LC/MS combination resp. [ Result] Both HPLC and LC/MS combination could detect melamine content better, but their detection limits were different. The detection limit of detecting melamine content by HPLC was 2.0 mg/kg and its peak flowing out was 7.25 min. The detection limit of detecting melamine content by LC/MS combination was 0.01 mg/kg. The cohen. -peak area curve of detecting melamine content by HPLC was y = 22. 351 3 ＋ 46. 380 7x （ R = 0. 998 4）. The concn. -peak area curve of detecting melamine content by LC/MS combination was y = 39. 702 5 ＋ 16. 264 6x （R = 0. 995 7 ）. [ Conclusion ] The detection by LC/MS combination needed shorter time, had higher sensitivity and better detection effect, but its cost was relatively high. The detection effect of HPLC was a little worse, but it was more economic and practical.