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分子生物学技术在植物病毒检测中的应用 被引量：3

Application of Molecular Biology Technology in Plant Virus Detection
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摘要 建立快速、高灵敏度、高通量的病毒检测体系是预防和控制植物病毒病的先决条件。综述了包括核酸杂交技术、反转录聚合酶链式反应、荧光定量PCR、DNA微阵列技术在内的分子生物学技术的特点及其在植物病毒检测和植物病毒病诊断中的应用。 The prerequisite of preventing and controlling plant virus disease is to establish a fast, sensitive and high - throughput virus detection system. The characteristics of the molecular biology technology including nucleic acid hybridization,reverse transcriptionpolymerase chain reaction,real-time PCR and DNA microarray technology and their applications in plant virus detection and plant virus disease diagnosis were reviewed.
作者 王新 莫笑晗 林良斌
机构地区 云南农业大学农学与生物技术学院 云南省烟草农业科学研究院
出处 《安徽农业科学》 CAS 北大核心 2009年第28期13498-13500,共3页 Journal of Anhui Agricultural Sciences
基金 云南省烟草公司科技项目(07A07)
关键词 植物病毒病 分子生物学技术 病毒检测 Plant virus disease Molecular biology technology Virus detection
分类号 S188 [农业科学—农业基础科学]
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参考文献31

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共引文献9

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二级引证文献24

安徽农业科学
2009年 第28期

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