黔产金钗石斛生物碱组成的HPLC指纹图谱建立

HPLC Fingerprints of Alkaloids in Guizhou Dendrobium nobile Lindl.

[目的]建立黔产金钗石斛生物碱组分的HPLC指纹图谱。[方法]流动相组成(水-乙腈),梯度洗脱条件0～50 min(5%～80%B)、50～60min(80%B),流速1.0ml/min,检测波长240nm,测定贵州4个产地金钗石斛色谱图。[结果]建立了贵州4个产地金钗石斛生物碱组分指纹图谱,所建立的指纹图谱可用于贵州金钗石斛产地和质量的鉴别。[结论]该方法稳定,重现性好,可为贵州金钗石斛质量控制提供参考。

[ Objective] The aim was to establish the fingerprints of alkaloids in Gnizhou Dendrobium nobile Lindl. by using HPLC. [ Method] With a mixture of A-water and B-acetonitrile as a mobile phase, gradient elution at 0 -50 min （5% -80% B） and 50 -60 min （80% B） respectively,flow rate at 1.0 mL/ min, detection wavelength at 240 nm, chromatograms of D. nobile Lindl. from four habitats in Guizhou were determined. [ Result]The fingerprints of alkaloids in D. nobile Lindl. from four habitats in Gnizhou could be used to identify the regions and the quality of D. nobile Lindl. [ Conclusion ] The method was stability,good reproducibility,and provided a reference for the quality control of D. nobile Lindl.