湖北地黄梓醇超声波提取及含量测定

Extraction with Ultrasonic and Determination of Catalpol Content in Rehmania henryi Brown

[目的]采用超声波法对湖北地黄中梓醇的提取工艺进行研究。[方法]在单因素试验基础上，采用L9（3^4）正交试验考察乙醇浓度、提取时间、料液比时湖北地黄中梓醇提取效果的影响；运用薄层色谱分析（TLC）法分别对湖北地黄根、茎、叶和生地黄中梓醇含量进行测定。[结果]优化的工艺参数为：乙醇浓度70％，提取时间30min，料液比1：40。TLC分析结果表明，张家界产湖北地黄叶中梓醇含量最高，为1．84％，其次是茎，为1．35％，根为1．29％，生地黄中梓醇舍量最低，为0．44％。[结论]为深入研究和开发湖北地黄提供依据，

[ Objective ] The research aimed to study the technology of ultrasonic of catalpol from Rehmannia henryi Brown. [ Method ] Based on the single -factor experiment, an orthogonal experiment of L9 （3^4） was made to study the effects of ethanol concentration, reaction time and the ratio of material to ethanol on the yield of catalpol. The TLC method was used to determine the contents of eatalpal in the Rehmannia henryi Brown＇s root,stem,leaf and Radix rehmanniae. [ Result ] The optimum extracting technology was as follow：the ethanol concentration 70% ,reaction time 30 min and the ratio of material to ethanol 1：40. The analysis result by TLC showed that the catalpa[ contents of Rehmannia henryi Brown＇s root,stem,leaf and Radix rehmanniae was in order：leaf（ 1.84% ） 〉 the stem（ 1.35% ） 〉 the root（ 1.29% ） 〉 the Radix rehmanniae （0. 44% ）. [ Conclusion ] The study can provide the basis for researching and developing deeply Rehmannia henryi Brown.