超声波提取酸枣仁总黄酮工艺研究

Study on the Supersonic Extraction Process of Flavonoids from Ziziphi Spinosae Kernels

[目的]探索超声波提取酸枣仁总黄酮的最佳工艺条件。[方法]采用3种提取方法(95℃索氏提取器中浸提提取2 h;60℃热回流浸提提取2 h;超声提取50 min)对酸枣仁总黄酮的提取效果进行分析,确定最佳提取方法。以乙醇浓度、料液比和提取时间为因子进行单因素试验和正交试验,确定超声波提取酸枣仁总黄酮的最佳工艺。[结果]用超声波提取总黄酮的提取率要高于回流提取和浸提法,超声波提取确定为理想的提取方法。影响总黄酮得率的主次因素为料液比>乙醇浓度>超声提取时间。超声波提取酸枣仁总黄酮的最佳工艺条件为:70%乙醇,料液比1∶30,超声提取50 min提取2次,提取率达到98.73%。[结论]采用超声波提取法的研究为酸枣仁总黄酮的工业化生产提供了参考依据。

[ Objective ] The study aimed to explore the optimum process condition for extracting flavonoids from Ziziphi Spinosae kernel with the supersonic extraction. [ Method ] The extracting effects on flavonoids from Z. Spinosae kernel by 3 kinds of extraction methods including the lixiviating extraction in Soxhlet extractor at 95 ℃ for 2 h,the heat refluxing extraction at 60 ℃for 2 h and the supersonic extraction for 50 min were analyze to confirm the optimum extraction method. With ethanol concn. ,solid-liquid ratio and extracting time as the factors, the single factor test and the orthogonal test wee conducted to confirm the optimum process condition for extracting flavonoids from Z. Spinosae kernel. [ Result ] The extraction rate of flavonoids from Z. Spinosne kernel by supersonic extraction was higher than that by refluxing extraction and the lixiviating extraction, so the supersonic extraction as confirmed as the perfect extraction method. The degree of the factors effecting the extraction rate of flavonoids was in order was solid-liquid ratio 〉 ethanol concn. 〉 extracting time. The optimum process condition for extracting flavonoids from Z. Spinosae kernel with the supersonic extraction was follows ：70% ethanol, solid-liquid ratio of 1：30, ultrasonic extracting time of 50 rain and extracting times of 2 times. Under this process condition, the average extraction rate was as high as 98.73%. [ Conclusion ] The study on supersonic extraction provided the reference basis for the industrialization production for of flavonoids from Z. Spinosae kernel.