摘要
用组织匀浆破碎和离心沉降法制备和分离出钝顶螺旋藻(Spirulinaplatensis)SpZ的单细胞或少于5个细胞的片段,并用60Coγ射线辐照处理,半致死剂量为12kGy。在24kGy的处理组中,筛选出4株藻丝长度、螺旋数、螺旋长分别为SpZ的15、10和18倍的超长钝顶螺旋藻突变体SpZ(E.L)。SpZ的生长曲线几乎呈直线型,而SpZ(E.L)的生长曲线则呈较明显的“S”形。在室外培养时,SpZ(E.L)的产量比SpZ高117%。
By mechanical grinding and sedimentating, the single cells and the fragments(less than 5 cells)of Spirulina platensis strains Sp Z were obtained.Then they were irradiaded with 60 Co gamma rays with their LD 50 of 1 2 kGy.After the single cells and the fragments were irradiaded with 2 4 kGy γ ray,a mutant [named Sp Z(E.L)] with extra long filaments was selected.The length of filaments,number of helix and length of helix of Sp Z(E.L) were 15,10 and 1 8 times of those of Sp Z,respectively.The growth curve of Sp Z was almost linear, while the growth curve of Sp Z(E.L) was S shaped.Moreover, the yield of Sp Z(E.L) was about 1 12 times of that of Sp Z cultured outdoors.
出处
《核农学报》
CAS
CSCD
1998年第3期146-150,共5页
Journal of Nuclear Agricultural Sciences
基金
浙江省科委"九五"重点项目
浙江省自然科学基金
关键词
钝顶螺旋藻
辐照
变异
生长特性
选育
pirulina platensis, irradiation, variation, growth characterization.