超长钝顶螺旋藻的选育及形态和生长特性初步研究

SELECTION OF THE MUTANT OF SPIRULINA PLATENSIS WITH EXTRA LONG FILAMENTS AND MORPHOLOGY AND GROWTH OF THE MUTANT

用组织匀浆破碎和离心沉降法制备和分离出钝顶螺旋藻（Ｓｐｉｒｕｌｉｎａｐｌａｔｅｎｓｉｓ）ＳｐＺ的单细胞或少于５个细胞的片段，并用６０Ｃｏγ射线辐照处理，半致死剂量为１２ｋＧｙ。在２４ｋＧｙ的处理组中，筛选出４株藻丝长度、螺旋数、螺旋长分别为ＳｐＺ的１５、１０和１８倍的超长钝顶螺旋藻突变体ＳｐＺ（Ｅ．Ｌ）。ＳｐＺ的生长曲线几乎呈直线型，而ＳｐＺ（Ｅ．Ｌ）的生长曲线则呈较明显的“Ｓ”形。在室外培养时，ＳｐＺ（Ｅ．Ｌ）的产量比ＳｐＺ高１１７％。

By mechanical grinding and sedimentating, the single cells and the fragments(less than 5 cells)of Spirulina platensis strains Sp Z were obtained.Then they were irradiaded with 60 Co gamma rays with their LD 50 of 1 2 kGy.After the single cells and the fragments were irradiaded with 2 4 kGy γ ray,a mutant [named Sp Z(E.L)] with extra long filaments was selected.The length of filaments,number of helix and length of helix of Sp Z(E.L) were 15,10 and 1 8 times of those of Sp Z,respectively.The growth curve of Sp Z was almost linear, while the growth curve of Sp Z(E.L) was S shaped.Moreover, the yield of Sp Z(E.L) was about 1 12 times of that of Sp Z cultured outdoors.