摘要
[目的]研究纤维素分解菌木霉、青霉、黑曲霉3种菌株之间的协同作用。[方法]将木霉、青霉、黑曲霉进行纯种发酵,添加纯种发酵粗酶液后测定CMC酶相对酶活力,采用混菌发酵观察滤纸分解度,测定CMC酶活力。[结果]混菌发酵的纤维素酶活明显高于纯种发酵的酶活力。[结论]青霉、木霉、黑曲霉3种菌株之间存在两两协同关系。
[ Objective] The aim was to study the synergetic effect of cellulose decomposition microorganisms. [ Method ] Three species of fungi strains were cultivated respectively and produced their crude enzyme liquid. CMCase relative activity was determined by adding crude enzyme liquid. The CMCase activity and filter paper breakdown were determined through the mixed fermentation. [ Result] The CMCase activity of the mixed fermentation is higher than that of the pure strain fermentation. [ Conclusion ] There were synergistic interactions between Trichiderma koningii and Penicillium spp. , between Trichiderma koningii and Aspergillus niger, between Penicillium spp. and AspergiUus niger.
出处
《安徽农业科学》
CAS
北大核心
2009年第27期12892-12893,12911,共3页
Journal of Anhui Agricultural Sciences
基金
国家"863"计划项目(2005AA001330)
国家农业科技成果转化资金项目(2006GB2G300314)
宁夏大学自然基金重点项目(2004-029)
关键词
纤维素分解菌
协同作用
混菌发酵
酶活力
Cellulose decomposition microorganisms
Synergetic effect
Mixed bacteria fermentation
Enzyme activity