纤维素分解菌协同作用研究

Study on the Synergetic Effect of Cellulose Decomposing Microorganisms

[目的]研究纤维素分解菌木霉、青霉、黑曲霉3种菌株之间的协同作用。[方法]将木霉、青霉、黑曲霉进行纯种发酵,添加纯种发酵粗酶液后测定CMC酶相对酶活力,采用混菌发酵观察滤纸分解度,测定CMC酶活力。[结果]混菌发酵的纤维素酶活明显高于纯种发酵的酶活力。[结论]青霉、木霉、黑曲霉3种菌株之间存在两两协同关系。

[ Objective] The aim was to study the synergetic effect of cellulose decomposition microorganisms. [ Method ] Three species of fungi strains were cultivated respectively and produced their crude enzyme liquid. CMCase relative activity was determined by adding crude enzyme liquid. The CMCase activity and filter paper breakdown were determined through the mixed fermentation. [ Result] The CMCase activity of the mixed fermentation is higher than that of the pure strain fermentation. [ Conclusion ] There were synergistic interactions between Trichiderma koningii and Penicillium spp. , between Trichiderma koningii and Aspergillus niger, between Penicillium spp. and AspergiUus niger.