小茴香对小鼠免疫功能的影响

Effect of Foeniculum vulgare Mill on the Immunological Functions in Mice

[目的]研究小茴香对环磷酰胺模型小鼠免疫功能的影响。[方法]以环磷酰胺制备小鼠免疫低下模型并结合体外药理试验,测定灌胃给药后小鼠体内巨噬细胞吞噬率、血清溶血素抗体水平,并观察植物血凝素诱导T淋巴细胞的增殖反应。采用含药血清体外培养小鼠腹腔巨噬细胞,MTT法考察巨噬细胞的活性,并观察体外培养的巨噬细胞吞噬红细胞的吞噬百分率和吞噬指数。[结果]小茴香提高免疫抑制小鼠碳粒廓清率,促进血清溶血素形成以及促进T淋巴细胞增殖。[结论]小茴香有提高小鼠免疫功能的作用。

[ Objective] The research aimed to study the effects of Foeniculum vulgare Mill on the immunological functions of the immunosuppressive mice induced by cyclophosphamide （CTX）. [ Method] Cyclophosphamide was used to prepare the immunosuppressive mice model. Based on the pharmacology test in vitro, the phagocytosis rate of macrophage and the serum hemolysin antibody level in mice after stomach administration were determined. The proliferative response of T lymphocyte induced by phytohemagglutinin （PHA） was measured. The serum containing drug was used to culture peritoneal macrophage （Mqb） in vitro. The activity of macrophage was studied by using MTI＇ assay. The phagocytic percent and phagocytic index of chicken red blood cell by macrophage cultured in vitro were measured. [ Result ] F. vulgare improved the carbon particle clearance rate of the immunosuppressive mice, promoted the formation of serum hemolysin and promoted the eration of T lymphocyte. [ Conclusion] F. vulgare could improve the immunological functions of mice.