氟比洛芬酯对手术致痛大鼠脊髓Fos蛋白表达的影响

Effect of flurbiprofen axetil on Fos-protein of spinal cord in incision-induced rats

目的:探讨氟比洛芬酯对手术致痛大鼠脊髓Fos蛋白表达的影响。方法:48只SD大鼠醉,按Brennan法手术,建立大鼠手术切口疼痛模型。20min后,对照(D)组大鼠经尾静脉注入生理盐水,另5组注入氟比洛芬酯注射液,K1组为2mg/kg,K2组为4mg/kg,K3组为8mg/kg,K4组为16mg/kg。每组在尾静脉注射后2h时,以3%戊巴比妥钠100mg/kg腹腔注射深麻醉下迅速切取脊髓L4-S1节段,SP法进行免疫组化染色,观察脊髓背角Fos样免疫反应(FLI)阳性细胞在脊髓上的变化情况。结果:外科手术刀口诱发的FLI阳性神经元主要位于与痛刺激同侧的脊髓浅层(Ⅰ-Ⅱ层),各剂量组同D2组比较,脊髓背角浅层FLI阳性细胞数目呈剂量依赖性减少(P<0.05)。结论:氟比洛芬酯可抑制脊髓Fos蛋白的表达,并呈一定的剂量依赖性。

Objective： To study the effect of flurbiprofen axetil on Fos-protein of spinal cord in incision-induced rats. Method： 48 250g-weight rats were randomly divided into 6 groups. The rats were established incision pain model by being operated according to Brennan＇ s method after having been anesthetized by breathing in isoflurane except control 1 group, which were anesthetized by breathing in isoflurane only.20minnutes later, control group I （D1 group） and control group2 （D2 group） were injected physiological saline by vena caudalis, the other 4 groups were injected flurbiprofen axetil 2mg/kg, 4mg/kg,8mg/kg, 16mg/kg（K1 ,K2,K3,K4group）re- spectively. 2hours after vena caudalis injection, all the rats were anesthetized deeply by pentobarbital sodium-perito injection 100mg/kg. After these rats were perfused, IA-S 1 sections of spinal cords were dissected, sliced by freezing and processed by immunostained with a rabbit polyclonal antiserum directed against c-los, then the changes of FLl positive neurons in the dorsal horn were studied. Result： Inci- sion-induced FLI positive neurons were mainly located in the superficial dorsal horn （laminae ] and II ）ipsilateral to the incision. Flur- biprofen axetil treatment markedly decreased the numbers of FLI positive neurons of dorsal horn in dose-dependent manner. Compared to the control 2 group, the differences were significant among the different dose group （P〈0.05）. Conclusion： The Fos expression of spinal cord were suppressed by flurbiprofen axetil administration in dose-dependent manner.