缺血性肠炎中肠神经肽的实验研究

The Change of Neuropeptide in Reperfusion after Ischemia Enteritis Model

目的:探讨肠神经系统中肠神经肽在应激状态下缺血性肠炎性反应中的变化。方法:取成年新西兰大白兔40只,随机分为对照组和实验组,各20只。采用夹闭肠系膜上动脉1小时后松夹恢复肠系膜上动脉血流制成肠缺血后再灌流模型,应用免疫组织化学的方法检测结肠壁肠神经组织中ENK(脑啡肽)、NT(神经降压素)、VIP(血管活性肠肽)、NPY(神经肽Y)的表达情况。结果:实验组ENK、NT的表达明显高于对照组(t=5.542、3.404P<0.01),而实验组VIP、NPY的表达与对照组无明显差异(P>0.05)。结论:在应激状态下缺血性肠炎中,肠神经组织ENK、NT的表达明显升高,而VIP、NPY的表达无明显变化。而ENK、NT为兴奋性神经递质,VIP、NPY为抑制性神经递质,所以在缺血性肠炎性反应中,肠神经系统以兴奋性作用为主。

Objective： To find the change of the neuropeptide in the rabbits whose intestine were in the condition of reperfusion after ischemia. Methods： We divided 40 rabbits into two groups：the control group （20）; the experimental group（20）. In the experimental group, exposing superior mesenteric artery （SMA）, clamping the initiation of SMA and unclamping it one hour later were carried by rou- tine.. In the control grOup, the difference was that there were no blockage of the portal veins. Then obtain the colon of expression of ENK.NT.VIP^NPY with the method of Immunohistochemistry in enteritis. Results： The expression of ENK.NT in the experimental group were significantly higher than in the control group （P〈0.01）, but the expression of VIP.NPY were not difference between the the experimental group and the control group （P〉0.05）. Conclusion： In the progress of enteritis, the expression of ENK and NT were signifi- cantly rise in the experimental group, but the expression of VIP and NPY were not obviously changed. The neuropeptide of enteric ner- vous system （ENS） effects to exciting in enteritis, because ENK andNT are excitatory neurotransmitter, and VIP and NPY are inhibi_ti.ve neurotransmitter.