4.1B蛋白表达与非小细胞肺癌进展的关系

The Relationship between 4.1B Protein Expression and Development of None Small Cell Lung Cancer

目的:探讨4.1B蛋白表达与非小细胞肺癌(NSCLC)进展的关系。方法:收集166例临床手术切除、石蜡包埋的原发NSCLC组织,免疫组化检测4.1B蛋白表达,分析其与NSCLC进展的关系。结果:26例4.1B蛋白表达于细胞膜上,69例4.1B蛋白表达缺失,71例胞浆表达。包括胞浆表达在内,4.1B蛋白异常表达率为84.34%。肿瘤最大直径≤3cm组、未转移组、临床分期Ⅰ和Ⅱ期组4.1B蛋白胞膜表达阳性率分别高于肿瘤最大直径>3cm组、转移组、Ⅲ和Ⅳ期组,差异有统计学意义(P<0.0.1),但4.1B细胞浆表达阳性率在各组间差异无统计学意义(P>0.05)。结论:在NSCLC组织内,4.1B蛋白存在表达缺失现象,也存在异常定位现象。表达于细胞膜的4.1B蛋白在NSCLC发展过程中可能发挥抑制作用,其表达缺失可能促进肿瘤进展。

Objective： To study the association of 4.1B protein expression with the development of None Small Cell Lung Cancers （NSCLCs.） Methods： To detect 4.1B expression with Immunohistochemistry （IHC）, tissues were collected from 166 cases of NSCLC who were surgical-resected and paraffin-embedded. The Association of the protein expression with the development of NSCLC was ana- lyzed too. Results： 4.1B protein of 26 cases expressed on the cell membrane, 69 casesshowed absence of 4.1B protein expression. 4.1B protein of 71 caseswas detected diffusely in the cytoplasm. A total of 140 cases （84.34%） had abnormal expression of 4.1B protein includ- ing these tumors with cytoplasm expression. The positive rate of 4.1B protein membrane expression in the group which tumor maximum diameter was ≤ 3cm was higher than that in the group which tumor maximum diameter was 〉 3cm. The positive rate of 4.1B protein ex- pression in the group NSCLC hasn＇t metastazed was higher than that in the group NSCLC has metastazed. The positive rate of 4.1B pro- tein expression in the group I ~ II stage was higher than that in the group III ~ IV stage. The rate of 4.1B protein cytoplasm expression has no significant difference between the group which tumor maximum diameter was ~〈3cm and the group which tumor maximum diameter was〉3cm, between the group NSCLC hasn＇t metastased and the group NSCLC has metastased, and between the group of I , II stage and the group of III, 1V stage. Conclusions： Loss of 4.1B protein expression and mislocalization of 4.1B protein exist in NSCLC tissues. 4.1B protein expressed at cell membrane may suppress the development of NSCLC. Loss of 4.1B protein expressed at cell membrane may pro- mote the development of NSCLC.