非洛地平对动脉粥样硬化大鼠主动脉NF-κB的影响

Effect of Felodipine on the Expression of NF-κB in Rat Model of Atherosclerosis

目的通过腹腔注射维生素D3与高脂饲料诱导相结合建立大鼠动脉粥样硬化模型,探讨非洛地平抗动脉粥样硬化作用的可能机制。方法30只雄性SD大鼠随机分为3组,每组10只,分别为正常组(正常饮食)、模型组(高脂饮食十腹腔注射维生素D360×104IU/kg)、非洛地平组〔高脂饮食十腹腔注射维生素D360×104IU/kg十非洛地平5mg/(kg·d)灌胃〕。喂养6周后采集主动脉标本,检测各组主动脉内皮细胞及平滑肌细胞核转录因子-κB(nuclear transcription fator-κB,NF-κB)的表达并进行比较。结果模型组和非洛地平组主动脉壁NF-κB阳性表达细胞数(分别为46.59±5.68、20.47±1.97)高于正常组(4.38±1.07,P<0.01),同时模型组阳性表达细胞数亦高于非洛地平组(P<0.01)。结论非洛地平抗动脉粥样硬化作用可能与其抑制血管内皮与平滑肌细胞NF-κB活化相关。

Objective To investigate the possible mechanism of felodipine on experimental atheroselerosis formation in rats. Methods The rat model of atherosclerosis was established by the methods of intraperitoneal injection of Vitamin D3 and high-fat diet. Thirty male SD rats were randomly divided into normal group, atherosclerosis model group and felodipine treating group. At the end of 6 weeks, the expression of NF-κB（nuclear transcription fator-κB） in endothelial cells and smooth muscle cells of aorta were determined. Results The positive rates of NF-κB in both atherosclerosis model group and felodipine treating group（46.59±5.68,20. 47±1.97） were higher than that of normal group（4.38± 1.07, P〈0.01）. The expression of NF-κB in atherosclerosis model group was higher than that of felodipine treating group （P〈0.01）. Conclusions The mechanism of felodipine in relieveing atheroselerosis may correlate with the inhibition of NF-κBp65 activation.