摘要
目的检测慢性阻塞性肺疾病(COPD)患者肺组织中白细胞介素(IL)-8的表达并分析其与COPD分级的相关性。方法采用PT-PCR和酶联免疫吸附试验(ELISA)检测对照组(n=15)、轻度COPD组(n=21)、中重度COPD组(n=15)患者肺组织中IL-8 mRNA和IL-8蛋白的表达,并分析其与COPD分级、肺功能损害和吸烟的关系。结果与对照组相比,IL-8 mRNA和IL-8蛋白在COPD患者肺组织中的表达增高,中重度COPD组高于轻度COPD组(P<0.05);IL-8 mRNA和IL-8蛋白在COPD吸烟组的水平高于COPD非吸烟组(P<0.05),但健康吸烟组和健康非烟组的表达无明显差异(P>0.05);IL-8的表达与COPD分级呈正相关(r=0.81,P<0.05),而与肺功能(FEV1/FVC%,FEV1%pred)呈负相关(r=-0.62,-0.56,P<0.05),与吸烟量(packs-years)呈正相关(r=0.53,P<0.05)。结论IL-8与COPD的分级密切相关,其表达水平可以作为临床观察与治疗的重要指标;吸烟可能是COPD患者肺组织IL-8增高的因素之一。
Objective To investigate the expression of interleukin (IL)-8 in lung tissues from patients with chronic obstructive pulmonary disease (COPD) and its association with stages of COPD. Methods The levels of mRNA and protein of IL-8 were measured with semi-quantitative polymerase chain reaction (RT-PCR) and enzymelinked immunosorbent assay (ELISA) in patients with mild COPD (n= 21), patients with advanced COPD (n= 15), and controls (n= 15). The correlations between IL-8 levels and stages of COPD, lung function (FEV1/ FVC%, FEV1% pred) and cigarette smoking were analyzed with Pearson correlation analysis. Results The levels of IL-8 mRNA and protein in the lung tissues of COPD patients were significantly higher than those in the control group (P〈0.05). The patients with advanced COPD had higher levels of IL-8 mRNA and protein than the patients with mild COPD (P〈0.05). The COPD patients who smoked had higher levels of IL-8 mRNA and protein than those who did not smoke (P〈0.05). But no significant differences in the levels of IL-8 mRNA and protein were found between smokers and and nonsmokers who did not have COPD (P〈0.05). Increased expression of IL-8 in patients with COPD was positively correlated with stages of COPD (r=0.81 ,P〈0.05); negatively correlated with lung function (FEV1/FVC%, FEV1% pred) (r = - 0. 62, - 0. 56, P 〈 0. 05), and positively correlated with volumes of cigarette smoking (r=0.53, P〈0.05). Conclusion IL-8 is associated with stages of COPD, which may serve as an indication for clinical progress. Cigarette smoking increases IL-8 expression in the lung tissues of COPD patients.
出处
《四川大学学报(医学版)》
CAS
CSCD
北大核心
2009年第5期885-888,共4页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金(批准号30370627)资助
关键词
慢性阻塞性肺疾病
IL-8
肺功能
吸烟
Chronic obstructive pulmonary diseases Interleukin-8 Lung function Cigarette smoking