肺组织白细胞介素-8与慢性阻塞性肺疾病分级的相关性研究

Association Between Interleukin-8 in Lung Tissues and Stages of Chronic Obstructive Pulmonary Diseases

目的检测慢性阻塞性肺疾病(COPD)患者肺组织中白细胞介素(IL)-8的表达并分析其与COPD分级的相关性。方法采用PT-PCR和酶联免疫吸附试验(ELISA)检测对照组(n=15)、轻度COPD组(n=21)、中重度COPD组(n=15)患者肺组织中IL-8 mRNA和IL-8蛋白的表达,并分析其与COPD分级、肺功能损害和吸烟的关系。结果与对照组相比,IL-8 mRNA和IL-8蛋白在COPD患者肺组织中的表达增高,中重度COPD组高于轻度COPD组(P<0.05);IL-8 mRNA和IL-8蛋白在COPD吸烟组的水平高于COPD非吸烟组(P<0.05),但健康吸烟组和健康非烟组的表达无明显差异(P>0.05);IL-8的表达与COPD分级呈正相关(r=0.81,P<0.05),而与肺功能(FEV1/FVC%,FEV1%pred)呈负相关(r=-0.62,-0.56,P<0.05),与吸烟量(packs-years)呈正相关(r=0.53,P<0.05)。结论IL-8与COPD的分级密切相关,其表达水平可以作为临床观察与治疗的重要指标;吸烟可能是COPD患者肺组织IL-8增高的因素之一。

Objective To investigate the expression of interleukin （IL）-8 in lung tissues from patients with chronic obstructive pulmonary disease （COPD） and its association with stages of COPD. Methods The levels of mRNA and protein of IL-8 were measured with semi-quantitative polymerase chain reaction （RT-PCR） and enzymelinked immunosorbent assay （ELISA） in patients with mild COPD （n= 21）, patients with advanced COPD （n= 15）, and controls （n= 15）. The correlations between IL-8 levels and stages of COPD, lung function （FEV1/ FVC%, FEV1% pred） and cigarette smoking were analyzed with Pearson correlation analysis. Results The levels of IL-8 mRNA and protein in the lung tissues of COPD patients were significantly higher than those in the control group （P〈0.05）. The patients with advanced COPD had higher levels of IL-8 mRNA and protein than the patients with mild COPD （P〈0.05）. The COPD patients who smoked had higher levels of IL-8 mRNA and protein than those who did not smoke （P〈0.05）. But no significant differences in the levels of IL-8 mRNA and protein were found between smokers and and nonsmokers who did not have COPD （P〈0.05）. Increased expression of IL-8 in patients with COPD was positively correlated with stages of COPD （r=0.81 ,P〈0.05）; negatively correlated with lung function （FEV1/FVC%, FEV1% pred） （r = - 0. 62, - 0. 56, P 〈 0. 05）, and positively correlated with volumes of cigarette smoking （r=0.53, P〈0.05）. Conclusion IL-8 is associated with stages of COPD, which may serve as an indication for clinical progress. Cigarette smoking increases IL-8 expression in the lung tissues of COPD patients.