高效阴离子色谱法快速分析保健食品中的腺苷

Determination of Adenosine in Food by High Performance Anion Exchange Chromatography

目的建立保健食品中腺苷的高效阴离子色谱测定方法。方法样品中的腺苷经乙腈-水提取后,以0.25mol/LNaOH为流动相,采用Amino PAC PA10氨基酸分离柱分离,Au为工作电极的脉冲安培检测器检测。结果腺苷在1.00μg/mL～20.00μg/mL浓度范围内线性关系良好,标准溶液测定的峰面积和保留时间的日内相对标准偏差(Related Standard Deviation,RSD)为4.97%和0.47%,日间RSD为6.08%和0.52%。将此法用于保健食品样品测定,取得较为满意的结果,样品平均加样回收率为93.3%～106.7%,样品测定结果的RSD为8.86%。结论本次建立的保健品中腺苷的高效阴离子色谱分析法,15min内可完成一次分析,方法简便准确,重现性好,适于保健品中腺苷的分析。

Objective To establish a high performance anion chromatography method for the determination of adenosine in food. Methods Adenosine in the food sample was extracted with Acetonitrile-water, and separated with Amino PAC PA10 Amino acid-separation column, with 0. 25 mol/L NaOH as the mobile phase. A pulse ampere detector with Au as working electrode was used throughout the experiment. Results A good linear result was produced for adenosine in the range of 1.00-20.00μg/mL. The inner-day RSD of peak area and retention time for the standard adenosine solution were 4. 97% and 0. 47%, respectively. The intra-day RSD of peak area and retention time for the standard adenosine solution were 6.08% and 0.52%, respectively. The proposed method was applied to food and satisfactory results were obtained, with and average recoveries of 93.3 %-106.7 % and 8.86 % of RSD. Conclusion The high performance anion exchange chromatography method is a simple, accurate, and reliable method for measuring adenosine in food, which can be completed within 10 minutes.