Kaposi肉瘤组织内人类疱疹病毒8型K1基因型的初步分析

C-enotyping of human herpesvirus-8 K1 gene in Kaposi＇s sarcoma

目的探讨新疆Kaposi肉瘤患者中人类疱疹病毒8型（HHV-8）基因型特征及分布情况。方法对11例本地Kaposi肉瘤冰冻标本进行组织基因组DNA抽提，应用巢氏PCR扩增K1基因。双向测序后绘制系统进化树，确定HHV-8基因型和分布特征。结果11例本地的Kaposi肉瘤标本中A、C基因型分别为3和8例，其中A5和C7亚型是国内首次分离出。基因型分布呈成簇聚集和来源一致的特点。AIDS相关型和经典型Kaposi肉瘤HHV-8基因型一致。结论新疆Kaposi肉瘤患者HHV-8基因型以A和C为主，并出现A5和C7亚型，与非洲和欧洲来源毒株的同源性很高。

Objective To profile the genotype of human herpesvirus 8 （HHV-8） in patients with Kaposi＇s sarcoma from Xinjiang Uygur Autonomous Region. Methods Genomic DNA was extracted from 11 frozen tissue samples of Kaposi＇s sarcoma. The HHV-8 K1 gene was amplified by nested-PCR and sequenced. Phylogenetic tree was drawn by Mega 3.0 software. Results Of the 11 tissue samples, 3 were positive for A subtype of HHV-8 gene, 8 for C subtype. A5 and C7 subtypes were first isolated and identified in China. HHV-8 genotypes in these samples tended to cluster and share common origin. AIDS-Kaposi＇s sarcoma-associated HHV-8 could share common genotypes with that associated with classic Kaposi＇s sarcoma. Conclusions A and C predominate in the genotypes of HHV-8 in patients with Kaposi＇s sarcoma in Xingjiang, which have a high homology with those strains from Africa and Europe. A5 and C7 genotypes of HHV-8 have been first isolated in China.