骨髓基质干细胞移植对大鼠脊髓损伤后BDNF与GAP-43基因表达的影响

Effects of mesenchymal stem cells transplantation on the brain-derived neurotrophic factor and growth associated protein-43 after the spinal cord injury of rats

[目的]研究骨髓基质干细胞(mesenchymal stem cells,MSCs)移植对大鼠脊髓损伤(SCI)后生长相关蛋白43(GAP-43)及脑源性神经营养因子(BDNF)基因表达的影响,探讨骨髓基质干细胞移植修复大鼠脊髓损伤的机制。[方法]大鼠SCI后第7d移植MSCs,应用RT-PCR法观察MSCs移植后,大鼠脊髓损伤区GAP-43和BDNF基因表达的变化。[结果]MSCs移植组较单纯损伤组明显增强了GAP-43 mRNA、BDNF mRNA的表达。[结论]MSCs移植后改变脊髓损伤区的微环境,上调BDNF mRNA,促进GAP-43 mRNA的表达,是修复脊髓损伤的机制之一。

[ Objective ] To observe the effects of mesenchymal stem cells （MSCs） transplantation on the brain-derived neurotrophic factor（BDNF） and growth associated protein-43 （GAP-43） after the spinal cord injury （SCI） of rats, and to investigate the mechanism of repairing the SCI by MSCs transplantation. [ Method ] Seven days after the operation of SCI, the MSCs were transplanted into the injured site. Then GAP-43 and BDNF were tested by RT-PCR. By means of above,the mechanism of repai- ring the SCI after the cell transplantation was investigated. [ Result] Compared with group B, transplantation of MSCs enhanced the expression of BDNF mRNA and GAP-43 mRNA in the spinal cord of group A, improved the neuron regeneration. [ Conclu- sion] The transplantation of MSCs can repair the injuried site and promote the regeneration of axon by enhanced the expression of BDNF mRNA and GAP-43 mRNA. It is one of the mechanism of repairing the SCI by MSCs transplantation.