结肠癌患者血清3＇sulfo-Lewis^a含量的测定和意义

Measurement of serum 3′sulfo-Lewis^a in patients with colorectal concer

目的测定结肠癌患者血清中3′sulfo-Lewisa的含量及其潜在的意义。方法采用酶联免疫吸附法(ELISA),建立人血清3′sulfo-Lewisa抗原的测定方法,对不同年龄、性别的非肿瘤健康人血清样本测定,通过与健康对照组的比较,探讨在结肠癌病人中血清3′sulfo-Lewisa含量变化的潜在意义。结果该方法在0~200ng/mL范围内具有良好的测定线性关系,因此血清标本需要稀释。样品溶血对测定结果没有显著影响,不同的包被时间会有一定的影响。不同年龄、性别的血清3′sulfo-Lewisa含量没有差别。健康志愿者的血清标本检测结果显示,血清3′sulfo-Lewisa含量的平均值为(9 160.25±504.14)ng/mL;95%的可信区间为(12 476.19-2 380.95)ng/mL。74例结肠癌病人血清测定结果显示,3′sulfo-Lewisa血清含量为(7 759±2 818)ng/mL,与正常相比显著降低(P=0.016 5),而且与转移相关,无转移组的病人3′sulfo-Lewisa血清含量为(8 007±2 543)ng/mL,转移组(6 867±1 722)ng/mL,两者相差显著,P<0.05。结论我们建立起了血清3′sulfo-Lewisa定量测定方法,初步观察了健康人群血清3′sulfo-Lewisa表达值,率先探讨了血清3′sulfo-Lewisa的潜在意义,研究结果具有临床应用价值。

Objective To investigate 3＇sulfo—Lewis^a antigen expression level in the serum of patients with colorectal cancer and its potential significance. Methods Enzyme linked immunosorbent assay （ELISA） was employed and a standard curve was established to measure serum 3＇sulfo—Lewis^a in 63 samples from 58 health volunteers of different ages and sexes. Results There was a correlation between the standard concentration and the detected value within the range from 0 to 200 ng/mL in our assay system, thus serum samples were diluted for the assay. Among the groups of different ages or sexes, there was no significant difference in 3＇sulfo—Lewis^a levels. There was also no significant difference between samples with and without hemolysis. Different coating times affected the detection levels. The expression level of serum 3＇sulfo—Lewis^a in health adult serum was （9 160.25± 504. 14） ng/mL. Interestingly, the level of 3＇sulfo—Lewis^a in patients with coloreetal cancer was （7 759 ± 2 818） ng/mL, which was significantly lower than that of the health subjects （P = 0. 016 5）. It further seemed to be related to metastasis tendency. In patients with and without metastasis, the contents of serum 3＇sulfo—Lewis^a were （8 007± 2 543） ng/mL and （6 867 ±1 722） ng/mL, respeeively. The difference reached significance （P〈0.05）. Conclusions Our study established a pioneer approch to measure 3＇sulfo—Lewis^a level in human serum and determin 3＇sulfo—Lewis^a level in patients with colorectal cancer, which is useful to observe the metastasis during the follow up.